FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10961547
Sequences flagged as poor quality0
Sequence length31
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA202105918.43771686605914Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT5020374.579983099100884No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA4052493.697005541279894No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2609902.3809595488665973No Hit
ACCGGGTGCTGTAGGCTTATCTCGTATGCCG2479812.2622810448196775No Hit
ACCGGGTGCTGTAGGCTTTATCTCGTATGCC2351982.1456642935527257No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1187281.0831317878762914No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1163311.061264436488755No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT756220.6898843748970834No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA726080.6623882559642357Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT642890.5864956835016079No Hit
CCTGGGAATACCGGGTGCTGTAGGCTTATCT572070.521888014529336No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA520490.4748326125865263No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT519650.4740662973939718No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT445960.40684038484713886No Hit
CGGGTGCTGTAGGCTTATCTCGTATGCCGTC438700.40021723211148935No Hit
CGGGTGCTGTAGGCTTTATCTCGTATGCCGT395290.3606151576962631No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT391830.35745866892693157No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG371040.3384923679112081No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT343380.3132587033563784No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG343190.31308537015806254No Hit
CCGGGTGCTGTAGGCTTATCTCGTATGCCGT341840.31185379217002857No Hit
CCGGGTGCTGTAGGCTTTATCTCGTATGCCG324950.29644538311973667No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA321950.29370854314632777No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT291680.26609382781463237No Hit
ACCGGGTGCTGTAGGCTTAATCTCGTATGCC291070.26553733702003923No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT272380.248486823985702No Hit
ACCGGGTGCTGTAGGCTATCTCGTATGCCGT265150.24189103964978664No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA262330.23931841007478233No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT261460.23852472648249376No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT258260.2356054305108576No Hit
TTCCGGCTCGAAGGACCAATCTCGTATGCCG240020.21896544347253175No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT225300.2055366820030056No Hit
CTGTGCGTGTGACAGCGGCTGAATCTCGTAT212680.19402370851486564No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT212020.1934216037207157No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT193610.17662652908389664No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT192380.17550442469479902No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA189130.17253951472360607Illumina Single End Adapter 1 (95% over 24bp)
CTCGTACGACTCTTAGCGGATCTCGTATGCC187650.17118934033672437No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT184930.16870793876083368No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG176930.1614096988317434No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTATG168710.15391075730460307No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT167550.152852512514885No Hit
TTTCCGGCTCGAAGGACCAATCTCGTATGCC164460.15003356734227385No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT164040.1496504097459966No Hit
CAGCATTGTACAGGGCTATGAATCTCGTATG156600.14286304661194266No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA147510.13457042149251378No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT144670.1319795463176867No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT142730.13020972313488233No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT141350.12895077674711425No Hit
CTCGTACGACTCTTAGCGATCTCGTATGCCG134140.12237323801102162No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC133040.12136973002077171No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA131330.11980973123592867No Hit
CGTTTCCGGCTCGAAGGACCAATCTCGTATG129190.11785745205489699No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT128850.11754727685791065No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT126510.11541254167865174No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT122350.11161745691552478No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCGT118400.10801395095053645No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT117060.10679149576241383No Hit
CTGGATGATGATAAGCAAATGCTGACTGAAC116420.10620763656808661No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT112590.10271360420203463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)