FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9366705
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA192541120.55590519825275Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT4719435.038516746283778No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT3504463.741401058323071No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT3125593.336915169208382No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT2068782.2086528827373124No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2044302.1825177583792805No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA1746731.8648286670712912No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA1619541.7290391872061734No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG1587471.6948008931636047No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1537171.6411000453200992No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT1195901.2767563406768976No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1153761.2317672009527363No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT1043191.1137214207130468No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC982351.0487679498820557No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT888030.9480708530908148No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA772370.8245909313894267No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT769560.8215909436669565No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT732030.7815234919857089No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA642500.6859402532694261Illumina Single End Adapter 1 (95% over 23bp)
TGTAAACATCCTTGACTGGAAGATCTCGTAT556170.5937733706783762No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT550540.5877627191205446No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT527500.5631649550188674No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA523270.558648959265825No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA422050.45058534457955063No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT413990.4419803975891202No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG412140.4400053167042199No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA382190.40803035859461784No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT364420.3890589059866837No Hit
CTTTCAGTCGGATGTTTACAGTTATCTCGTA337470.3602867817444875No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG293100.31291686884555453No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT283500.3026678004698557No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT283310.30246495432492004No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT282400.3014934280518069No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT272460.2908813718378021No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT258640.27612698382195233No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT256650.2740024373565731No Hit
GTAAACATCCTTGACTGGAAGCATCTCGTAT248530.26533343368879453No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG236530.252522098219171No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC227270.24263601768177817No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATGC222680.23773568186464716No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG211410.22570370263609243No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA210370.22459338689539168No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT208490.22258627767181735No Hit
CTTTCAGTCGGATGTTTACAGCAATCTCGTA196140.2094012782509965No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG188890.20166109640476562No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA185850.1984155580857943Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTTATCTCGTA184770.1972625378935282No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT178620.19069672846534616No Hit
CTAAGCCAGGGATTGTGGGTATCTCGTATGC171180.18275370047417955No Hit
TGTAAACATCCTACACTCTCAGCTATCTCGT154210.1646363368975536No Hit
TGTAAACATCCTACACTCTCAGCATCTCGTA152060.16234097262591274No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT149540.1596505921772918No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC144470.15423780294137587No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA138550.14791754410969493Illumina Paired End PCR Primer 2 (95% over 24bp)
TGAGGTAGTAGGTTGTATAGATCTCGTATGC138350.14770402185186787No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA136440.14566488428961946No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG135670.1448428235969853No Hit
GTAAACATCCTTGACTGGAAGCTGTATCTCG135200.14434104629109168No Hit
GTAAACATCCTTGACTGGAAGCTGATCTCGT129020.13774320852423558No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA127620.13624855271944616No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT119860.1279638891157563No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT115880.12371479618499782No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCGT107200.11444793019530347No Hit
CTTTCAGTCGGATGTTTACATCTCGTATGCC106820.11404223790543205No Hit
GTTCTACAGTCCGACGATCTGGTCAAATCTC99570.10630205605920119No Hit
TCTACAGTCCGACGATCTGGTCAAATCTCGT95150.10158321416122318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)