FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17732273
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA236174413.31890164334826Illumina Single End Adapter 1 (95% over 24bp)
CACCCGTAGAACCGACCTTGCGATCTCGTAT8611194.856224579894524No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT6831613.8526420160573887No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA6632703.7404680155781493No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC4696242.648413996333127No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT4665842.6312701141021235No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG3460681.9516279723417296No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT3130311.7653179600832898No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG3119641.7593006829976054No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2789421.5730752622633322No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2624721.480193768728916No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2033101.1465535185477913No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1338750.7549793531827532No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1278170.7208156562895236No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT1154360.650993812242796No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1128650.6364948250007205No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA1060780.5982199800330167No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT1028940.5802640191700184No Hit
CGTACCGTGAGTAATAATGCGATCTCGTATG1014690.5722278243742356No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA968970.5464443278083977No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG948900.5351259818749688No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT940670.5304847269157204No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA929450.5241572809080934No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT866160.48846529714492887No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA853380.4812580992859742No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA843480.4756750586910093Illumina Single End Adapter 1 (95% over 23bp)
GTAGTGTTTCCTACTTTATGGATCTCGTATG819370.46207838104003923No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT756840.4268149943326498No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT708480.39954268694148803No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT687420.38766603694856266No Hit
GTACAGTACTGTGATAACTGAAATCTCGTAT571050.3220399325004753No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT569640.3212447721733136No Hit
TTCCGGCTCGAAGGACCAATCTCGTATGCCG513950.28983875896789996No Hit
AAGTTTCTCTGAACGTGTAGAGCATCTCGTA458530.2585850105059853No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA447950.2526184883348006No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT410880.23171310299587652No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT403520.22756247887679146No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT376560.21235856226666486No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT371860.20970802784279263No Hit
TACAGTACTGTGATAACTGAAAATCTCGTAT353470.1993371069800245No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT349650.19718284283126025No Hit
TCGTATCCGGCTCGAAGGACCAATCTCGTAT327010.1844151621171183No Hit
TGGTTAGTACTCTGCGATCTCGTATGCCGTC318540.1796385607192039No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT315820.17810463441432467No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG314810.17753505148493937No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTAT314410.177309474087163No Hit
CACCCGTAGAACCGACCTTGCGTATCTCGTA313160.17660454471911188No Hit
TGGTGTAATGGTTAGCACTCTGGATCTCGTA313120.17658198697933422No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT312760.1763789673213355No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG305260.17214939111302877No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT302490.1705872676334275No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCGT299590.16895183149954887No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT296910.16744046293444725No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT290900.16405116253285745No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA290420.1637804696555258No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT269980.15225346462915387No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG268470.1514019099525481No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT267490.150849245327996No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT266730.15042064827222093No Hit
TTTCCGGCTCGAAGGACCAATCTCGTATGCC266600.1503473356179436No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA264800.14933223732795No Hit
CAGCATTGTACAGGGCTATGAATCTCGTATG236170.13318653508210707No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA232560.13115069906717544No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA232390.13105482867312046No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG232070.13087436675489938No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA231380.13048524574373516No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT228740.1289964349184112No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA225040.12690984398897986Illumina Single End Adapter 1 (95% over 24bp)
CATTATTACTTTTGGTACGCGATCTCGTATG221720.12503755158743607No Hit
CTGGATGATGATAAGCAAATGCTGACTGAAC213740.1205372825017977No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT213370.12032862340885458No Hit
ATCCCACCAGAGTCGCCAATCTCGTATGCCG211900.11949962647202646No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT211010.11899771676197406No Hit
GGCTGTAGGAATACTTTTATCTCGTATGCCG202580.11424367310383728No Hit
TAAAGTGCTTATAGTGCAGGTAGAATCTCGT201140.11343159447184238No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA192560.10859295928953946No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC191040.1077357651779893No Hit
TGGTTAGCACTCTGGAATCTCGTATGCCGTC189970.10713234563893755No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA189640.10694624428577205No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT184330.10395170433029088No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA184000.10376560297712539Illumina Paired End PCR Primer 2 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)