FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11465400
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA157101013.70218221780313Illumina Single End Adapter 1 (95% over 24bp)
CACCCGTAGAACCGACCTTGCGATCTCGTAT6337275.527299527273362No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT4428173.862202801472256No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA3662253.1941755193887698No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2321372.0246742372703963No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1291571.126493624295707No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1268151.1060669492560224No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG1258521.0976677656252727No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT1007830.8790186125211507No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA901860.7865927050081113No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT808690.7053308214279485No Hit
TGGTAGACTATGGAACGTAGGATCTCGTATG736650.642498299230729No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA685720.5980776946290579No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT603980.5267849355452056No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG603410.5262877876044447No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT581400.507090899576116No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT537960.469202993353917No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT519650.45323320599368533No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA493750.43064350131700596No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT488330.42591623493292863No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA467470.40772236467981926Illumina Single End Adapter 1 (95% over 23bp)
TGAGAACTGAATTCCATAGGCTGTATCTCGT464720.4053238439129904No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT448940.39156069565824136No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT441360.3849495002354911No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA425250.3708985294887226No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA370090.32278856385298377No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT326230.2845343380954873No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT282280.2462016152947128No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA280670.2447973904094057No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA276500.24116036073752334No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT260120.2268738988609207No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA245330.21397421808223002No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT243460.21234322396078636No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG242830.21179374465784012No Hit
ACCATCGACCGTTGATTGTACATCTCGTATG222390.19396619394002826No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG219690.19161128264168714No Hit
TCGGATCCGTCTGAGCTTGGCTATCTCGTAT211280.18427617004203953No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG209420.18265389781429345No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT202670.1767666195684407No Hit
CACCCGTAGAACCGACCTTGCGTATCTCGTA202610.17671428820625534No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA200060.17449020531337764No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA196690.17155092713730005No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT195690.17067873776754408No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT193790.16902157796500777No Hit
TGTGACTGGTTGACCAGAGGGGATCTCGTAT193110.16842848919357373No Hit
AGCAGCATTGTACAGGGCTATGAAGCTCGTA190090.16579447729691071No Hit
ATCTCGTATGCCGTCTTCTGCTTGGAAAAAA189480.16526244178135957Illumina Single End Adapter 1 (95% over 24bp)
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT188770.16464318732883285No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG182680.15933155406701904No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT181020.15788371971322412No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT178360.1555636959896733No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC177610.15490955396235628No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT175230.15283374326233712No Hit
ACGAGAACTTTGAAGGCCGAAGATCTCGTAT174630.15231042964048355No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT166160.14492298567865056No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT161730.14105918677063164No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT160460.13995150627104158No Hit
AACATTCATTGCTGTCGGTGGGTATCTCGTA157140.1370558375634518No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA156210.13624470144957873No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT149970.13080223978230152No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT146370.12766235805118006No Hit
ACCACTGACCGTTGACTGTACCATCTCGTAT144680.1261883580162925No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA144270.12583076037469254No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA142740.12449631063896595No Hit
CTTCTCACTACTGCACTTGACTAATCTCGTA141760.12364156505660509No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT141040.1230135887103808No Hit
AACATTCATTGCTGTCGGTGGGTTATCTCGT140470.1225164407696199No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCA136870.11937655903849845No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG136570.11911490222757165No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA135200.11792000279100599No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA134250.11709142288973781Illumina Single End Adapter 1 (95% over 24bp)
TCGTATCCCACTTCTGACACCAATCTCGTAT129010.11252115059221658No Hit
CAGCATTGTACAGGGCTATGAATCTCGTATG125190.10918938719974881No Hit
CTGGCGGAGCGCTGAGAAGACGGATCTCGTA123260.10750606171611982No Hit
CTCGTACGACTCTTAGCGGTGGATCATCTCG122290.10666003802745651No Hit
TGAGGTAGGAGGTTGTATAGTTATCTCGTAT119310.10406091370558376No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT118390.10325849948540829No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT114760.10009245207319413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)