FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17522999
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA194833511.118730304099202Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT9460015.3986249728143No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT9014625.144450444812557No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT5966283.4048281347273948No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA5316813.034189524293187No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT3489111.9911603030965188No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2899101.654454240395722No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2365121.3497232979354732No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA2227441.2711522725076911No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG1996521.1393711772739357No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1928391.1004908463442815No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT1679790.95862015400446No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1388740.7925241563958315No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1298500.7410261222979011No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA968170.5525138704852977No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT905510.516755151329975No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT892310.5092221942145863No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA841020.4799520903927461No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC782680.4466587026570052No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG740920.42282716560104805No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT687700.39245565214036704No Hit
TGGTAGACTATGGAACGTAGGATCTCGTATG651630.37187127614399795No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT635400.3626091629634859No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA633440.3614906329675645No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT625320.3568567229844617No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT588390.33578156341845367No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA581920.3320892730747745Illumina Single End Adapter 1 (95% over 23bp)
GTAGTGTTTCCTACTTTATGGATCTCGTATG576560.3290304359430712No Hit
CGGGTCGGAGTTAGCTCAAGCGGTATCTCGT570250.3254294541704876No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA549110.31336530921447864No Hit
TCAGTGCACTACAGAACTTTGTCATCTCGTA478780.2732294854322596No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA464490.2650744886762819No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT457100.26085717404880293No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA430140.24547167981919077No Hit
AAGTTTCTCTGAACGTGTAGAGCATCTCGTA409960.23395538628975554No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT397820.22702734845787528No Hit
CGTACCGTGAGTAATAATGCGATCTCGTATG394160.22493866489406295No Hit
AGCAGCATTGTACAGGGCTATGAAGCTCGTA388380.2216401427632336No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG387690.22124637455038376No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT371630.21208127672666077No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT363810.20761857031436226No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA359430.20511899818061963No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT353970.2020030931919816No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT347100.19808253142056337No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT333540.19034413002020945No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT332480.18973921073670094No Hit
CGGGTCGGAGTTAGCTCAAGCGGATCTCGTA330060.1883581685988797No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT317940.18144154433838636No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA313660.17899904006157852No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA310690.17730412471061605No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTAT307730.17561491614534705No Hit
TCGGATCCGTCTGAGCTTGGCTATCTCGTAT297250.16963420473858384No Hit
TGGTGTAATGGTTAGCACTCTGGATCTCGTA297140.1695714300959556No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTAT286560.16353365083225765No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT286520.16351082368948375No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG280100.15984706727427195No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT278240.15878560513528536No Hit
ATCTCGTATGCCGTCTTCTGCTTGGAAAAAA277470.15834618263688768Illumina Single End Adapter 1 (95% over 24bp)
TGTAAACATCCTTGACTGGAAGCATCTCGTA254540.14526052304174644No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT238740.13624380164605385No Hit
CAACACCAGTCGATGGGCTGTCATCTCGTAT234010.13354449201303956No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG232480.13267135380193767No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT231900.13234036023171605No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG231710.13223193130354No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT226460.12923586881446492No Hit
TATGGCACTGGTAGAATTCACTGATCTCGTA224880.12833419667489565No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA224520.12812875238993052No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG222700.12709011739371784No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT213250.12169720491338269No Hit
AAGTTTCTCTGAACGTGTAGAGTATCTCGTA211020.12042459170373747No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG208960.11924899385088135No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG207360.11833590813992514No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG204420.1166581131460431No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT203830.11632141279012799No Hit
CACCCGTAGAACCGACCTTGCGTATCTCGTA202490.11555670350720217No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA196580.11218399316235766Illumina Single End Adapter 1 (95% over 24bp)
TTTGGCAATGGTAGAACTCACAATCTCGTAT191120.10906808817371957No Hit
TGTGACTGGTTGACCAGAGGGGATCTCGTAT183040.10445700533339071No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT182590.10420019997718427No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG180330.10291046641045863No Hit
ACCACTGACCGTTGACTGTACCATCTCGTAT177900.10152371748694389No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT175350.10006848713510741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)