FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12771304
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA160765412.58801763704004Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT6758105.291628795305475No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA4653103.6434024278178643No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA3269942.560380678433463No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2930802.294832226998903No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT2060931.6137193194994028No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1699821.3309682394217537No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1420301.1121025699490044No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG1035500.8108020919398677No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1016330.795791878417427No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT998260.7816429708352413No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT981310.768371029301315No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT794320.6219568495119997No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT774330.6063045715613691No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT752820.5894621254023864No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG585030.4580816492975189No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA565230.44257814237293236Illumina Single End Adapter 1 (95% over 23bp)
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA562930.4407772299523996No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT501360.3925675874601372No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG497850.38981923850532413No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT488090.3821771057990633No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT463470.3628995128453602No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT442320.34633894863046094No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT440340.34478859793800226No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA438580.34341050843359455No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT413410.3237022625097641No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT408270.31967761475257345No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT403090.3156216467793735No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT383070.29994587866673594No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA379310.29700177836186503No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA368620.28863145063338874No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA364510.28541329843843666No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT363710.2847868941182513No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT360200.2820385451634383No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG336810.2637240488520201No Hit
AGCAGCATTGTACAGGGCTATGAAGCTCGTA333870.26142201297533907No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG323820.25355280870301106No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA321650.2518536869845084No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT314480.2462395382648475No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT305940.2395526721468693No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT275960.21607817024792458No Hit
ACTGGACTTGGAGTCAGAAGGCATCTCGTAT266420.20860829872971468No Hit
GTGGGCACATTACCCGTCTGACCATCTCGTA255560.200104860083199No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT249250.19516409600773735No Hit
CTAAGCCAGGGATTGTGGGTATCTCGTATGC249130.19507013535970955No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA237840.1862300043910943No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC229630.17980153005519248No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT228800.17915163557300023No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG221300.1732790950712629No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT220650.1727701415611123No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA219280.17169742416279496No Hit
ATCTCGTATGCCGTCTTCTGCTTGGAAAAAA211310.16545687112294877Illumina Single End Adapter 1 (95% over 24bp)
TAACTGGTTGAACAACTGAACCCATCTCGTA211300.16544904106894645No Hit
TTTGGCAATGGTAGAACTCACACATCTCGTA210940.16516715912486304No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT200670.15712569366448406No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTAT200490.15698475269244236No Hit
ATCACATTGCCAGGGATTTCCAGATCTCGTA198780.15564581345804626No Hit
CAAAGTGCTGTTCGTGCAGGTAGAGCTCGTA194090.15197351813095986No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT188170.1473381261615885No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT185500.14524750174297002No Hit
ATCACATTGCCAGGGATTTCCATCTCGTATG183370.14357970024047662No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA182220.14267924403021023No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA169350.13260196452922896No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTAT167260.13096548324274482No Hit
TGGTGTAATGGTTAGCACTCTGGATCTCGTA157540.12335467075249323No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG153520.12020698904356203No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA151920.1189541804031914Illumina Single End Adapter 1 (95% over 24bp)
TATGGCACTGGTAGAATTCACTGATCTCGTA149780.1172785488466957No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC149680.11720024830667251No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG144830.11340267211554904No Hit
ACCACAGGGTAGAACCACGGACATCTCGTAT140810.11025499040661785No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT138470.10842275777007578No Hit
TGTAAACATCCCCGACTGGAAGCTATCTCGT138470.10842275777007578No Hit
CGTTTCCGGCTCGAAGGACCAATCTCGTATG134870.10560393832924188No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT134400.105235925791133No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA133490.1045233908769222No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT133370.1044294302288944No Hit
ATGGCACTGGTAGAATTCACTGATCTCGTAT133120.1042336788788365No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA132790.10397528709676004No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG129140.10111731738591453No Hit
AAGTTTCTCTGAACGTGTAGAGCATCTCGTA129040.10103901684589138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)