FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9278805
Sequences flagged as poor quality0
Sequence length31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA156596716.87681765054875Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT4703515.069090254617917No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA3131283.374658697968111No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT2643032.84845947296015No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1741231.876567079489223No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA1468481.5826175892261989No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT1083491.1677042463981082No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1048531.130026980845055No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT999661.0773585607198342No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT990811.067820694582977No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT937671.0105503887623462No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA730540.7873212121604021No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG712810.7682131481370715No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG619530.6676829613296109No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA521660.5622060168308312Illumina Single End Adapter 1 (95% over 23bp)
AACATTCAACGCTGTCGGTGAGAATCTCGTA505750.5450594122842327No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT493180.531512409194934No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT481640.5190754628424673No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT476040.5130402029140606No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA393470.42405245071967784No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT378230.4076279219145138No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA337280.36349508368803957No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT305400.32913721109560984No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT300770.32414734440480214No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT295710.31869405596949174No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG293840.3166787102433988No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA287320.30965194332675383No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA282660.30462974488632966No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT280890.30272217165895826No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT252900.2725566492667968No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG243770.2627170201335194No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT243080.26197338989234065No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA238820.2573822814468027No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT235970.25431076523323853No Hit
AACATTCATTGCTGTCGGTGGGAATCTCGTA232680.2507650500252996No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT230640.24856649105138No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT227520.2452039890912677No Hit
ATCTCGTATGCCGTCTTCTGCTTGGAAAAAA218990.23601099495031957Illumina Single End Adapter 1 (95% over 24bp)
AACATTCATTGCTGTCGGTGGATCTCGTATG209630.2259234890699826No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA209400.22567561232292305No Hit
TCCAGCATCAGTGATTTTGTTGAATCTCGTA199090.21456426770473136No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT190150.20492940631902493No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT189990.20475697032107046No Hit
AACATTCATTGCTGTCGGTGGGTATCTCGTA184720.19907735963844483No Hit
AGCAGCATTGTACAGGGCTATGAAGCTCGTA179300.19323609020773688No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC178060.19189971122358968No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC174500.18806301026910255No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC168050.18111168410156264No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT165730.17861136213122272No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA165160.1779970588885099No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG164350.17712410164886536No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT160530.17300719219770216No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG159180.17155226346496127No Hit
TGAGAACTGAATTCCATAGGCTGATCTCGTA153330.16524757228975068No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT151740.16353398956007806No Hit
TCTACAGTCCGACGATCTGGTCAAATCTCGT150810.16253170532196765No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT149800.16144320308488003No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT144530.1557635924022544No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA143750.15492296691222632No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA137850.1485643894876549Illumina Single End Adapter 1 (95% over 24bp)
CTACAGTCCGACGATCTGGTCAAATCTCGTA133820.14422115778917652No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT129490.13955460859453345No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT120540.12990896995895485No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA115630.12461734027172681No Hit
ATCACATTGCCAGGGATTTCCATCTCGTATG113470.12228945429934135No Hit
TGTAAACATCCCCGACTGGAAGCTATCTCGT111990.12069442131826243No Hit
ATCTCGTATGCCGTCTTCTTCTTGAAAAAAA111210.11985379582823434Illumina Paired End PCR Primer 2 (95% over 24bp)
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT109830.11836653534587699No Hit
TTCTACAGTCCGACGATCTGGTCAAATCTCG108850.11731036485840579No Hit
ATCACATTGCCAGGGATTTCCAATCTCGTAT103490.11153375892693079No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT103240.11126432768012691No Hit
CACCCGTAGAACCGACCTTGCGTATCTCGTA102860.11085479218498502No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA100990.10883944645889207Illumina Paired End PCR Primer 2 (95% over 24bp)
GGAATACCGGGTGCTGTAGGCTTATCTCGTA96810.10433455601233134No Hit
GTTCTACAGTCCGACGATCTGGTCAAATCTC95680.10311672677677784No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA94900.10227610128674974No Hit
ATCTCGTATGCCGTCTTCTGCTTGCAAAAAA93040.10027153281052895Illumina Single End Adapter 1 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)