FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12533461
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA168161313.416988332273105Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT5161924.118511239632851No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT2961772.3630902908621967No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA2779002.217264648607436No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2521302.011655040854238No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2128821.6985092944398996No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT2078171.6580974720390482No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT1803011.4385571551225953No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA1336381.0662497772961514No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1144570.9132114425536569No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1124610.8972860728572898No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT990390.7901967381555661No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA873790.6971657708912167No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT661920.5281222800310306No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT650630.5191143930634962No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA599210.4780882152184461No Hit
CTCCCACTGCTTCACTTGACTAGATCTCGTA575270.4589873459533643No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT546380.43593704883272066No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA509210.4062804360264096No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG492180.3926928084748499No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA491630.3922539831575652Illumina Single End Adapter 1 (95% over 23bp)
TGTAAACATCCCCGACTGGAAGATCTCGTAT462900.36933134431104064No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT435180.3472145483198934No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT408270.32574402234147454No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT396980.3167361353739402No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT389430.31071226056394163No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA386750.30857398447244544No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT360990.2880210023392581No Hit
TCCCACTGCTTCACTTGACTAGATCTCGTAT359750.2870316507148345No Hit
CTTCTCACTACTGCACTTGACTAATCTCGTA349260.278662055117896No Hit
CCCCCACAACCGCGCTTGACTAGATCTCGTA347630.27736153645030687No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT340460.27164085004134136No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA329420.26283242912711824No Hit
TTCTCACTACTGCACTTGACTAATCTCGTAT288690.23033541972165547No Hit
CCCCCCACTGCTAAATTTGACTGATCTCGTA284420.22692853953109998No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG277580.22147114831250525No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG268960.21459355879433462No Hit
CTCCCACTGCTTCACTTGACTAATCTCGTAT260770.2080590508878593No Hit
CCCCCCACAACCGCGCTTGACTAGATCTCGT257210.20521865428870767No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA246320.1965299130064712No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT227560.18156198036599788No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG216880.17304079056854288No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT204240.16295578691312798No Hit
ATCTCGTATGCCGTCTTCTGCTTGGAAAAAA203070.1620222857836315Illumina Single End Adapter 1 (95% over 24bp)
CTAGACTGAAGCTCCTTGAGGATCTCGTATG198060.15802498607527482No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT190820.1522484491713821No Hit
TTCTCACTACTGCACTTGACTAGTATCTCGT190110.15168196557997826No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA188470.15037346827025672No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT187530.14962347590980657No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA185350.14788413192493277No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT183740.14659957054160858No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC176410.14075122585852384No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT174640.13933900620108045No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT170870.13633105811714738No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT168370.13433639758403526No Hit
AACATTCATTGCTGTCGGTGGGATCTCGTAT166700.13300396434791634No Hit
TCCCACTGCTTCACTTGACTAATCTCGTATG165100.1317273816067246No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT162580.12971676378934757No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG161330.1287194335227915No Hit
TCGTTTCCCGGCCCATGCACCAATCTCGTAT156300.12470617653016992No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA154280.12309449081941533Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATATCTCGT147790.11791635207545625No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT146020.11650413241801286No Hit
CGTGGGCACATTACCCGTCTGACCATCTCGT145210.11585786240528453No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT145050.11573020413116536No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT141240.1126903414787025No Hit
AACATTCATTGCTGTCGGTGGATCTCGTATG137130.10941111956226615No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG132580.1057808373920021No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTATG125770.10034738209980468No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT125680.10027557432061264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)