FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10710372
Sequences flagged as poor quality0
Sequence length31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA165970115.496203119742248Illumina Single End Adapter 1 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTATCTCGTAT4739494.4251404152908975No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA4466144.169920521901573No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT3553363.3176812159278875No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT2045431.9097655991780678No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1606641.500078615383294No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1399051.3062571496116102No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1278691.1938801005231192No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1138201.062708186046199No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG910610.8502132325562548No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG743510.6941962426701892No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG710740.6635997330438196No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT673110.6284655659019127No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA637210.5949466554476353No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT632230.5902969570057883No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT607060.5667963727123577No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA488980.45654810122374834Illumina Single End Adapter 1 (95% over 23bp)
TGTAAACATCCCCGACTGGAAGATCTCGTAT480720.44883595079610683No Hit
TAGGTAGTTTCCTGTTGTTGGGATCTCGTAT460120.42960225844629857No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT416380.38876334080646313No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA389770.3639182653973177No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT383970.35850295395902215No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT380330.35510437919429877No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA377290.3522660090611232No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT321590.300260345765768No Hit
TGAGAACTGAATTCCATAGGCTGTATCTCGT318840.29769274120450717No Hit
AGCAGCATTGTACAGGGCTATGAAGCTCGTA309890.28933635545058567No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT289920.2706908779639027No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTAT272070.2540247901753553No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT264360.24682616065996588No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT263870.2463686602108685No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG236590.22089802296316133No Hit
TCGTATCCCACTTCTGACACCAATCTCGTAT231040.21571613012134402No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT228770.21359668926532152No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG227190.21212148373557896No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT219840.20525897699911824No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA216440.20208448408701396No Hit
ATCTCGTATGCCGTCTTCTGCTTGGAAAAAA203170.18969462498594822Illumina Single End Adapter 1 (95% over 24bp)
GTACAGTACTGTGATAACTGAATCTCGTATG196380.1833549759055988No Hit
TCGTTTCCCGGGCGGCGCACCAATCTCGTAT192930.18013379927419887No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC187270.17484920224993117No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA182070.16999409544318347No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT182070.16999409544318347No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT181190.16917246198358002No Hit
ACTGCAGTGAAGGCACTTGTAGATCTCGTAT177530.16575521373113836No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA172570.1611241887770098No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT170730.15940622790692985No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT168820.15762290982983598No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG162820.15202086351435787No Hit
AAGTTTCTCTGAACGTGTAGAGCATCTCGTA162760.15196484305120306No Hit
ACCACTGACCGTTGACTGTACCATCTCGTAT149710.13978039231503817No Hit
ACTGGTGCGGACCAGGGGAATCCGAATCTCG148150.13832386027301385No Hit
TTCCGGCTCGAAGGACCAATCTCGTATGCCG145220.13558819432228872No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA143480.13396360089080006Illumina Single End Adapter 1 (95% over 24bp)
TTTCCGGCTCGAAGGACCAATCTCGTATGCC142530.13307661022418268No Hit
TGTAAACATCCCCGACTGGAAGCTATCTCGT140090.13079844472255492No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT133100.12427206076502291No Hit
CTCGGCGTGGCGTCGGTCGTGGATCTCGTAT132720.12391726449837598No Hit
ATCTCGTATGCCGTCTTCTTCTTGAAAAAAA126640.11824052423202482Illumina Paired End PCR Primer 2 (95% over 24bp)
TAGGTAGTTTCCTGTTGTTGGGTATCTCGTA126610.11821251400044741No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG119800.11185419143237975No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT119610.11167679329905628No Hit
CAAAGTGCTTACAGTGCAGGTAGAATCTCGT119560.11163010957976063No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT117850.11003352637984937No Hit
TCGTTTCCCGGCCCATGCACCAATCTCGTAT117430.1096413831377659No Hit
CAGCATTGTACAGGGCTATGAATCTCGTATG116250.10853964736238852No Hit
TGGTGTAATGGTTAGCACTCTGGATCTCGTA114990.10736321763613814No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA113590.10605607349585991No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT113360.10584132838709989No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA109070.10183586527153306Illumina Paired End PCR Primer 2 (95% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGCAAAAAA107340.10022060858390354Illumina Single End Adapter 1 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)