FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007772631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12901175
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1380291.0698947964042036Clontech SMART CDS Primer II A (100% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT1123190.8706106226758416Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT346680.2687197096388507TruSeq Adapter, Index 27 (97% over 39bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT277450.21505793076987173Clontech SMART CDS Primer II A (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)