FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007772635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19045447
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1966551.032556494998516Clontech SMART CDS Primer II A (100% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT1592650.8362366081510191Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT477230.2505743236165578Clontech SMART CDS Primer II A (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT291030.1528081750982269TruSeq Adapter, Index 6 (97% over 36bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT227210.11929885394656266Clontech SMART CDS Primer II A (100% over 24bp)
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT216090.11346018814890509Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)