FastQCFastQC Report
Fri 6 Jan 2023
EGAF00007772640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345255601
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCAGATCAGTACGTACGAGTCCCATACGATGTACTCGCAGTAGTCC11950310.3461293593901754No Hit
AGTACTCTCTCAGTACGTACGAGTCCCGGTGGTTGTACTCGCAGTAGTCC11625490.3367212571303079No Hit
ACACAGAACTCAGTACGTACGAGTCTTGATGAGTGTACTCGCAGTAGTCC11579830.3353987586721294No Hit
TCATCTGTCCAGTACGTACGAGTCTCACTACGTGTACTCGCAGTAGTCCG10055180.29123872200410733No Hit
ACGCTATGTAGTACGTACGAGTCGTACGCGATGTACTCGCAGTAGTCCGA8619800.2496643059528526No Hit
TACCTCACTGTCAGTACGTACGAGTCACAGCGTATGTACTCGCAGTAGTC8270190.2395381849286784No Hit
ACTATCCTGTCAGTACGTACGAGTCACCACGGATGTACTCGCAGTAGTCC8121790.2352399201193553No Hit
CCATGCGTCAGTACGTACGAGTCGCCTTCCATGTACTCGCAGTAGTCCGA8104970.23475274482223388No Hit
CATCCGACACAGTACGTACGAGTCCCACGTTCTGTACTCGCAGTAGTCCG7159950.20738113963283683No Hit
TCGACGTTGGTCAGTACGTACGAGTCTAGCCTTGTGTACTCGCAGTAGTC7135320.20666775511630295No Hit
GGTGTGACCGTCAGTACGTACGAGTCGGTGAGCGTGTACTCGCAGTAGTC7092050.20541448073423144No Hit
ACCATTCACTCAGTACGTACGAGTCCTGGTACATGTACTCGCAGTAGTCC6564520.19013507618664238No Hit
AACGCATTGGTCAGTACGTACGAGTCATGCGGATTGTACTCGCAGTAGTC6268800.18156982774046293No Hit
ACTGGTGCTCAGTACGTACGAGTCCTGTCTAGTGTACTCGCAGTAGTCCG6210410.17987861694385662No Hit
GCGTCGTCTCAGTACGTACGAGTCATAGTCAGTGTACTCGCAGTAGTCCG6131360.1775890089035804No Hit
CACCTACGCCAGTACGTACGAGTCAACGTTCTTGTACTCGCAGTAGTCCG6031270.17468999728117374No Hit
ATGAGTGTGTCAGTACGTACGAGTCACGACATCTGTACTCGCAGTAGTCC5785380.16756802737575285No Hit
GACGTGGTCTCAGTACGTACGAGTCCCATGATGTGTACTCGCAGTAGTCC5618800.16274319616323907No Hit
ATTGCCGACCAGTACGTACGAGTCAGTTGCCTTGTACTCGCAGTAGTCCG5475420.1585903308777893No Hit
AAGCTGGTCTCAGTACGTACGAGTCGTCCTTCATGTACTCGCAGTAGTCC5434970.15741873511271434No Hit
CCGCAACACCAGTACGTACGAGTCCTGCTGCTTGTACTCGCAGTAGTCCG5305870.1536794764409919No Hit
CCATTCTGCGTCAGTACGTACGAGTCTTGAGCTCTGTACTCGCAGTAGTC5296150.15339794588879097No Hit
TGACTGAGACAGTACGTACGAGTCCTTCTGAGTGTACTCGCAGTAGTCCG5261650.15239868621276909No Hit
GCAGCGATAGTCAGTACGTACGAGTCGCTTACGTTGTACTCGCAGTAGTC5225910.15136351111650756No Hit
ACATGGATTTCAGTACGTACGAGTCCTCCTATCTGTACTCGCAGTAGTCC4988860.14449758340053692No Hit
TCATCAGAGCAGTACGTACGAGTCCGCCACCATGTACTCGCAGTAGTCCG4974270.1440749979317497No Hit
CCAATGCCTAGTACGTACGAGTCTTAGCGTCTGTACTCGCAGTAGTCCGA4949070.14334510390752503No Hit
GAGACATTGAGTACGTACGAGTCTAAGCCATTGTACTCGCAGTAGTCCGA4937870.14302070656342517No Hit
CACAACCAGGTCAGTACGTACGAGTCATCCTGCCTGTACTCGCAGTAGTC4845500.14034529739605875No Hit
GGATACAATAGTACGTACGAGTCAGAAGTCCTGTACTCGCAGTAGTCCGA4825330.1397610925361932No Hit
AGCATTGTTGTCAGTACGTACGAGTCCGACGTGATGTACTCGCAGTAGTC4668890.13522995677628413No Hit
GTCCGCTCAAGTACGTACGAGTCCCAATGCCTGTACTCGCAGTAGTCCGA4638530.13435060826138487No Hit
TTCCGCCAGGTCAGTACGTACGAGTCCTACGGTATGTACTCGCAGTAGTC4624930.1339566972006922No Hit
TAGTAACGGCAGTACGTACGAGTCTGAGATCATGTACTCGCAGTAGTCCG4461640.12922715770800774No Hit
GGATAGTCAGTCAGTACGTACGAGTCCTGGTACATGTACTCGCAGTAGTC4446240.12878111135987044No Hit
TGAACGTCAAGTACGTACGAGTCCCTCCTTGTGTACTCGCAGTAGTCCGA4363420.12638230885644633No Hit
ACAGAAGAGAGTACGTACGAGTCCTGGTACATGTACTCGCAGTAGTCCGA4321190.1251591570848984No Hit
CAGTGCCATTCAGTACGTACGAGTCTCGAACGCTGTACTCGCAGTAGTCC4309540.12482172591893738No Hit
TGAGCTAGACAGTACGTACGAGTCCGCGAGTGTGTACTCGCAGTAGTCCG4291750.12430645549469305No Hit
TGATTCACCCAGTACGTACGAGTCGACAATGTTGTACTCGCAGTAGTCCG3976750.11518278019188456No Hit
CATTCAGTTCAGTACGTACGAGTCCCATATGCCGTACTCGCAGTAGTCCG3894320.11279527366740677No Hit
TGTACACGACAGTACGTACGAGTCGACCGTAGTGTACTCGCAGTAGTCCG3884790.11251924628443609No Hit
AACTGCGACAGTACGTACGAGTCTGAGTGACTGTACTCGCAGTAGTCCGA3824150.11076286637852402No Hit
GTCGCAGGTTCAGTACGTACGAGTCGGTGGAGCTGTACTCGCAGTAGTCC3820490.11065685796071995No Hit
GATACTGTGGTCAGTACGTACGAGTCGGACAGATTGTACTCGCAGTAGTC3812070.11041298067167346No Hit
CTTACCTAGGTCAGTACGTACGAGTCCTTAGGACTGTACTCGCAGTAGTC3641560.10547432074823893No Hit
AGGTATTCCTCAGTACGTACGAGTCAATACGTCTGTACTCGCAGTAGTCC3547390.10274677629342789No Hit
ATAAGTTCGTCAGTACGTACGAGTCCCAATGCCTGTACTCGCAGTAGTCC3543510.10263439578493616No Hit
TCTAATACCCAGTACGTACGAGTCCCATTCTGCGTACTCGCAGTAGTCCG3521840.1020067448522001No Hit
CCTCGAGAGTCAGTACGTACGAGTCAGCAAGTCCGTACTCGCAGTAGTCC3481520.10083891441344062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)