FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007772643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52083416
Sequences flagged as poor quality0
Sequence length39-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGGGAATTGTTGAAGGACAGCATCAGCATGTAGCGTGCTAAAATAGC10441852.004832017930621No Hit
AGCAAACCCAACATGAACTACGATAAGCTCAGCCGCGCCCTCCGTTACTA8177391.570056388006501No Hit
GAAGACTTATCTCCTGCCTTTGATCATTCACCAAACAAAATAATGACTGA7285911.3988924996778245No Hit
CAATTTACTTACCCCAGCAAGACATTTTTCCAGTGTATCCAATATAATCA6403661.2295007685363801No Hit
GCCATATAGATAAATCGGTACTGTGCTTCTGTCTGGACCATCCCTGACCT6276641.2051129672446985No Hit
CCAAGTTTCCAGAAAACAGAAATCGAAACAGATACAGAGATGTAAGCCCA6131281.1772038915419833No Hit
GTCCCTGTCCGGCCAATTCCAGCACTGCGGGCAGAAGGACAAAAAGCAGG5745781.1031880090199921No Hit
TGCTTGTAAGAGAGAGACCCAGATCCTTGGAGGATCTCCATCAGAAAAAA5720571.0983476967025358No Hit
TTGTAGTAGTGGCGCAGGGCTCTGGACATTTTCTCATAGGTCATGTTTGT5410251.0387663512700474No Hit
AACGTGCCCAACTTTACTCGGCTCAGGGGCTCGGACACCCCAGACATCCT5334871.024293414241493No Hit
TGGAGGAACATGCCGAGGAATTGAATTTCCAAGATGCAAAAATACTTGAA4845420.9303191633974238No Hit
TCCACTCGGGCCACAAAGTTACTGGGCACATAGCCTTCTCTTCCTGTGAC4767080.9152779072709055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4740090.9100958354958898No Hit
GCTCTCTGTCAATCTCTCTGGTTGTGCTCAGAACTCCAGCAGTGCTCAGA4722070.9066360009873393No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCCGTGGCCCCTGCACCA4719340.9061118418192846No Hit
TCGTACAAATTTACTCACCTGCAAACAGCCGATAGGTTCGGTGGCATTTA4679930.8985451338291637No Hit
CTTGTGCCTTCTGCTTCTCCCCTGCTGTTGCAGAGAGCCCTGCTGACGAA4651180.8930251425904936No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATTGAGCAC4598490.8829086786473452No Hit
GGCTTCCGAATGGTAAACTGAAATGTGACGTCTGTGGCATGGTTTGCATT4483920.8609112735616267No Hit
CCCGAATGACCCCCAGATTTGACTCCAACAACGTGGTCCTCATTGAGGAC4301920.8259673290246554No Hit
CAGCAACTCTGTGCCATTCCAGAGATCGCTAACAGTGAGGAGGTGCAGGA4140040.7948864183562767No Hit
GCGTGTAGTAAATAGAATCCCTCAGCAGGCACCAGGAGGAAAGAGCCACA4125570.7921081827658925No Hit
GTGGTCTCAGCTAATTTGCTTAGTTCTCGAACTCTGCTTTCTATTTCAGC4116640.7903936254872377No Hit
GCTTTGGGACTTGAGATTGTTTTTGTCTTTTTTCTGGCATTATTTCTGCT4094340.7861120322829823No Hit
CTGTTTGTTGGACATACTGGATACAGCTGGACAAGAAGAGTACAGTGCCA4093480.7859469125450604No Hit
TGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTGAAAGA4069670.7813753998009654No Hit
CCAAGCCAAAACGACTGCAGGAGTGGTACAAAAAGATGCTGGACAAGGCG4021410.7721094945078103No Hit
TTTCTCTTTTGTTATTAGGAACAATCTCAGTTACAAAGTGAACTACTAAA3932910.7551175214774699No Hit
GTTCCATGTAATACTGGACCAACTCAGCCAAAGTGGCAAATTTCTCCCCT3908660.7504615288674614No Hit
CTAAAGAGGGAGCAACGCGAGGGTGGCTTACGGGTTGGTGGTGCTGAAGA3856460.7404391447749894No Hit
TTTCTCTTTTGTTATTAGGAACAATCTCAGCTACAAAGTGAACTACTAAA3851810.7395463461920393No Hit
GCATTCACCATAAATATCCAGGTTTGGTGTAGGCATAAGATACACGTTGA3824690.73433931445664No Hit
TGTGACTTTGGATTGGCTCGAGATATCATGAGTGATTCCAACTATGTTGT3735460.7172071816487613No Hit
GCTTTCAAAAGATTACATCAAATATGGACTCTGTGGGTCGAATACAAATG3691630.7087918350055995No Hit
ATCTCCTTTGTCCCCGCCACCTGAGGAATTGCCCACTTCCCCGCCACCTG3668060.7042664021883664No Hit
CCGGCTGTCAGAAAGCAACTGATAGACGTAATCCCAAAGCAGTCTACAGT3575150.6864277105019378No Hit
CATTCACACCAGAGTCATCATCCGTGGCTAAAATGGCTGCTACCAAAGAA3531260.6780008438770606No Hit
CCCATTTCTTCTCCTTACTGCAGGTGAACGCCCCTTCCACTGTAACCAGT3475060.6672104610035563No Hit
TGGCTCTGACTGTACCACCATCCACTACAACTACATGTATAACAGTTCCT3456170.6635835867601311No Hit
TCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAATATTTCACCCTTCA3436290.6597666328184004No Hit
GGAACACCAGGCAGATTCATTTACACAGGTAAGAACCACAGCACATGGTG3426200.6578293558932463No Hit
CGTGAAACTGCTTCAGATGCTGCTCCATGGTCTCAATGATGCTCTTTTGC3420060.6566504777643617No Hit
CCATGATGACCACCAGTCCTACCAAGGACACCCTGCCTTAAATCCCAAGA3411820.6550684002754351No Hit
AAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATTGATGCCAAAGA3310390.6355938711854077No Hit
TCTCATCAAAGGAATGCACTAGGATTCGCGCGGGGGTCTTTCTGGTTCCT3199300.6142646250391871No Hit
GCTATCCCAACAGATTGCCAAGTATGCCCACGGACATGGAACACACAGGA3181620.6108700704270242No Hit
AGAGACAGCGGGATTCCTTCCCACACCAGGATGAGAATCACAATCACTGC3136930.6022896040459405No Hit
TGTACACACCGATGTCATGGTCGTTCCTCAGGGTGCAGGTGAGGCGGATT3132200.6013814454873697No Hit
AGGAATACCGGCCACGAACGTTCTGGTTTAACATGGCCGACGCCGCCTTC3076990.5907811423121709No Hit
TTTACTTACTCTCGTCTCCACAGACACATACTCCATAATTTAAAACCAAA3059790.5874787475537319No Hit
GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCG2928840.5623363874596858No Hit
TGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGG2892380.5553360785705761No Hit
GCTATCCCAACAGATTGCCAAGCATGCCCACGGACATGGAACACACAGGA2889190.5547235995427028No Hit
TGGGACATCACCCTCACTTACTGGGTCACACTTGATCACTCTGGAGAGGA2720770.5223870108673363No Hit
AGCAAGAGAAACTGGGCACAGTTTACTGTCAGGCAAGCTTTCCTGGAGCA2666170.511903827506245No Hit
CGGTCTGAAGAGCCAAGAGCTATTTGATGAAATTAGAATGACCTACATCA2576060.49460273496653906No Hit
TTCTCCTTCCACCAGTTCCACGTTGCGCATCTCCTCCATGTAGCAGGCAT2495900.479212039394651No Hit
GTGGGTATTAAACAAGAACCAGATGACGGGAGCTATTACCCAGAGGCCAG2457240.4717893311759736No Hit
ATCATGGCCCGTCACCTCCACCCCATCCAGACCAGCTTCCAGGAGGCTAC2444580.4693586150340062No Hit
TGATATGGAGGATGCTGGTAACAGACGTGGTCCCATTTGGGTGAGACAGA2388360.4585643921665968No Hit
CCGGACAAAAGTACAGCAGCCACCCAACCAACCAGTCCCAGCTTCCAGTC2361460.4533996003641543No Hit
TTCTCCTTCCACCAGTTCCACATTGCGCATCTCCTCCATGTAGCAGGCAT2328520.447075130402353No Hit
GTGGCAGCGCTCTTTATGTTCCTCTAAAGAGCTTCGCTGTTTATAGCTTC2249030.43181307462628793No Hit
GGTTTCATTTTGCCAATTGAAGTTTATTTTAAAAACAAGGTATGTAATCT2248460.4317036347999908No Hit
ACAGTTCTCTGAATGCCAATATGATTACTTCCAGATTAAAATGCAAAATT2244020.430851156153045No Hit
GTGAGTGGATGGGTAAAACCTATCATCATAGGTCGTCATGCTTATGGGGA2214750.42523132507284084No Hit
CCCATCTGCCCAAGAGACTCCGTCAAAGTGATCAACAAGGAAGGGGAGGT2208680.42406588692262426No Hit
TACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT2154250.41361534350972684No Hit
TTCCTGGAGCAGCATTTGAATTACTGTCCTGTGACATAGCACGGACTTCC2106790.4045030379727781No Hit
GGTGTTTGTCTCCTCTTCATTGTCGTTTTAACCCTGCTAATTTGTCACAA2053240.3942214542917077No Hit
ACACACATGGCAAAGACAAAGAAAGGAAAGCGTCTCTCGACCAAGGGAAA1925070.36961285335047916No Hit
GCTCCCCCTACTATTATAGCGCTGCCGCCCGAGGAGCCGCCCCACCTGCA1891380.36314438361723433No Hit
AGCCTGTCTGAAGTGCAGTCTCTGAGCTCCTTCCAGTCCGAATCGTGCGA1765680.3390100219232932No Hit
CTTGCTGTGTTTGTCTCCTGAGGCATGGGACCCAGGGTGAGGATATCCCA1752890.3365543458209423No Hit
AGGACCATTGCCTCAAGGGCATGAATTTGAACTGTACGAAGTGAGATTTC1738170.3337281103067433No Hit
GCTCATGACTGCTAAGCTGAGCGCCTTGCTTGTGGCTGGAGCCTGGATGG1692920.32504012409631505No Hit
GTCACTCTTGGAGCTTTGCTGTCCTCCCGAGGTGGTGGAGAGGTCCTCGG1683370.32320652700660035No Hit
GGGATAGAGGAGCGGATGACGGTCGTCTGGGACAAGGCGGTGGTAAGGCT1639850.31485070026896855No Hit
GCTTCTTACCAGCATTCTGTGTGCTCCTGATGCAGCGAAGGCGTATCCCT1579350.3032347187058545No Hit
CTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGATGT1531010.2939534534370787No Hit
AGTAACTTGAGGGGCACGACCGAGGGGGTTTACTACCAGGCCCCCGACTT1511140.2901384194923006No Hit
GTCACAGTCAGCAGTAGCATATGCATGAGGTACAGTACTTTTAGATTCAG1495340.2871048243072229No Hit
CTAGGTGGTGCTGAGAGAGGTGTGGGGTGGCTGAGTGGTCCAGAAGCAGC1469360.28211667222441783No Hit
AGTAACTTGAGGGGCACGACTGAGGGGGTTTACTACCAGGCCCCCGACTT1462910.2808782741900032No Hit
CATCACCAGGTGCGTTACCTGTTGGAATGGACAGAGGAGGACCTGGAGGA1427750.274127564904729No Hit
ATTATGAGACAGGAAGCATCAAGCCTGGGGTAATTGGAGGATCCAAACCA1424860.27357268578543315No Hit
GGAAGCCACGAGAATATTGTGAACCTGCTGGGGGCGTGCACACTGTCAGG1414430.2715701289638913No Hit
TCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAGCCAAGCCGAGT1385210.2659598978684501No Hit
TCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAACCAAGCCGAGT1380770.2651074192215042No Hit
AGCTTATTGAGTTCATCATTAAAGTCATCTATTTTCAACCTGGGGTACAA1361130.2613365452066354No Hit
ATGGTGTGGTTGACCTGGCCCAGCCCCTCGCTGCTCTCTGAGTGGGTGCA1358620.26085462597153763No Hit
AGCTTATTGAGTTCATCATTAAAGTCATCGATTTTCAACCTGGGGTACAA1273520.24451545190507473No Hit
CTTTAGCATTTACTAACATTCCAGATGTAAAAAGTGCCTCAGAACGACCC1256830.2413109769912173No Hit
AGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCG1219640.2341705083245692No Hit
TCTCGAGAACTCTGTGTGCCACTGGGAGTGCCTCTAGACCGTTCATATCC1182040.22695131978286523No Hit
AGGTTAAGGTTGGCAGTGATACTAAGCTGGCTCTGCAGTGCAGGAGGGTA1118630.21477661910655016No Hit
GTGCTCATGTGCGGAAACAACAACTGCTGCAGGTGAGGCTGTTGTGGCCT1117510.21456157944786108No Hit
TGCAGTACACCGGATTGGCCGCACCGGGCGCTCGGGAAACACAGGCATCG1104100.21198686353445018No Hit
GAAAGACGTCACACTGAGAGTATCTGGAGACCTTCATGTTGGAGGAGTGA1101480.21148382433287405No Hit
AGATGTACCTATGGTCCTAGTAGGAAATAAATGTGATTTGCCTTCTAGAA1087450.2087900686084031No Hit
TGTCCAGCACGAGAGATCTCTCTGATCCTATCTTGCACAGGTCTTCTGCC1068230.20509983446554275No Hit
CAACATCTCCTTCGCAGCCGCGCAAAAAAAGTAGGCCATTTATTCATTAC1034110.19854880486333693No Hit
GATTCACCAGGTGCTCATGGACCGGGAGGACACGTGTCACCGTACCTGCT988550.18980129874737864No Hit
TCCACCTGCCTTCAGAAGCCCAGGAGGTGGCCCTTGTGGCCTATAACTCA986740.18945377929896148No Hit
GATTCACCAGGTGCTCATGGACCATGGAGTCCCATGTCATTACACCTGCT972530.18672546362934414No Hit
CTCACTCACCACTGCAGCTGGCTTTGGAGGCAGCTCAGAGTTAGAGGTCT897220.17226596657945784No Hit
AACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACAATC829500.1592637472165804No Hit
ACAATGAGCTTTCACCCGAAAGGGAGCACTCCAATATGGCAATTGACCTC803420.1542563951642496No Hit
GCTTCACTGCCGTTCTTGGCCATTCCCTAGGAATAGAGGAAAAGAACAAG746860.1433968924004524No Hit
TTGGAGAGCAGCAGCAGGTTCTCCACGGCGCTGTCCTGGGCCGAGTGGTT729450.14005417770600914No Hit
GTCTCTCTCTCTCTCTTTAGGCCAAGGCAGGTGGATCACCTGAGGTCAGG697170.13385642754307822No Hit
CGTGAAACTGCTTCAGATGCTGGAGAGATGAGCTGCTGCGACTACTGCGA692550.13296938895098587No Hit
ATTTGCCAACAAGGACAGTTGATACAAAACAAGCCCACGAACTGGCCAAG676190.1298282739365636No Hit
CAGGAAATTACACACTGCCATGATGAAGATGATGATGAAGAGGAGGAGGA639790.12283948502916936No Hit
TGCTTGTAAGAGAGAGACCCAGGTCCTTGGAGGATCTCCATCAGAAAAAA636520.12221164602567543No Hit
CAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGA614980.1180759725898163No Hit
TAAGCTTGCCTTGGGAGCCCCCCAGGCATGTTGGAATTTCCAGTGTATTG559600.10744302946642363No Hit
TCGTACAAATTTACTCACCTGCAAAGAGCCGATAGGTTCGGTGGCATTTA544900.1046206339461298No Hit
ACATCCGAGTTAGAACTGAGGTTTGTTAATCTGGGGTCCGCATTGATTTC536370.10298287654557833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)