FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007772644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242354406
Sequences flagged as poor quality0
Sequence length137-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAGTGTAGTACGTACGAGTCACTCGAGTCGTACTCGCAGTAGTCCGA163966336.765560102918037No Hit
CCGAGCTGATCAGTACGTACGAGTCCATGCCAGTGTACTCGCAGTAGTCC58373992.4086209515827828No Hit
TGCCGAACTTCAGTACGTACGAGTCGTCTGACGTGTACTCGCAGTAGTCC53078522.190119869328887No Hit
TCTCAGGATTCAGTACGTACGAGTCGCCTTCCATGTACTCGCAGTAGTCC14479380.5974465345598049No Hit
AATATCGCTCAGTACGTACGAGTCTGAGCAGATGTACTCGCAGTAGTCCG12881970.5315343844006698No Hit
GATGTTACAAGTACGTACGAGTCTCGTGACGTGTACTCGCAGTAGTCCGA12515500.5164131408446521No Hit
ACAACGAGCTCAGTACGTACGAGTCCGGTTCTATGTACTCGCAGTAGTCC11091820.4576694182320745No Hit
GATTAGTGTAGTACGACTCGAGTCGTACTCGCAGTAGTCCGACTGAGATA10112460.4172591770417411No Hit
AGTCTAAGCTCAGTACGTACGAGTCACCATACTTGTACTCGCAGTAGTCC9622790.3970544690654397No Hit
ACAGGTATCAGTACGTACGAGTCAATATCAGCGTACTCGCAGTAGTCCGA8148980.33624228808119955No Hit
GGTACTTCCAGTACGTACGAGTCCACACTGCCGTACTCGCAGTAGTCCGA8046230.33200262924041907No Hit
AAGTTGCCAAGTACGTACGAGTCCATTCAGTTGTACTCGCAGTAGTCCGA7928020.3271250616339115No Hit
CGTGGTACCAGTACGTACGAGTCTATGACCGAGTACTCGCAGTAGTCCGA7162340.29553166035694023No Hit
TTGCGAACAAGTACGTACGAGTCGGTGAGCGTGTACTCGCAGTAGTCCGA6686700.27590585664863054No Hit
CCGCTCATGTCAGTACGTACGAGTCTAGCACTATGTACTCGCAGTAGTCC6628130.27348914795466933No Hit
TACTAGTAGCAGTACGTACGAGTCGCCTTCCATGTACTCGCAGTAGTCCG6181480.25505952633681434No Hit
GCACATGTTCAGTACGTACGAGTCTATACGGATGTACTCGCAGTAGTCCG5750550.23727854157518388No Hit
CTTAAGTTGTCAGTACGTACGAGTCTCTACAGTTGTACTCGCAGTAGTCC4887940.2016856256370268No Hit
AAGTTCTCTAGTACGTACGAGTCCCGTCCACTGTACTCGCAGTAGTCCGA4674770.19288982928579396No Hit
CGTCAGCACTCAGTACGTACGAGTCTGGATTAGCGTACTCGCAGTAGTCC4654030.1920340577591975No Hit
CTAGTTCCAGTCAGTACGTACGAGTCATTAGCGGTGTACTCGCAGTAGTC4593210.18952450982054767No Hit
AGTACTCTCTCAGTACGTACGAGTCACCATGTCTGTACTCGCAGTAGTCC4562850.18827179894554918No Hit
ATTGATTGGTCAGTACGTACGAGTCCATAGAGATGTACTCGCAGTAGTCC4546140.18758231282166168No Hit
ATTCACCTCTCAGTACGTACGAGTCTGACCTCCTGTACTCGCAGTAGTCC4544170.18750102690520098No Hit
GTGAGGACGCAGTACGTACGAGTCGCTTACGTTGTACTCGCAGTAGTCCG4531150.1869637971426028No Hit
GGTGAGTTCAGTACGTACGAGTCACTGCTAGTGTACTCGCAGTAGTCCGA4493170.18539667069225885No Hit
AGTGACGATCAGTACGTACGAGTCGCGCTACCTGTACTCGCAGTAGTCCG4467640.18434325472919194No Hit
TTATTCGACGTCAGTACGTACGAGTCCCATATCTTGTACTCGCAGTAGTC4099080.16913577383033013No Hit
CACATTCTGTCAGTACGTACGAGTCTCCAGCAGCGTACTCGCAGTAGTCC3727670.1538106965548627No Hit
AGAGCCGCATCAGTACGTACGAGTCCTATTCCGTGTACTCGCAGTAGTCC3699180.15263514540767212No Hit
CCTTGCTTGTCAGTACGTACGAGTCGCGGTAAGTGTACTCGCAGTAGTCC3685030.15205128971329698No Hit
CCATATGCCAGTACGTACGAGTCGACATATGTGTACTCGCAGTAGTCCGA3650060.1506083615414031No Hit
TCAGACAAGCAGTACGTACGAGTCAGGTCACGTGTACTCGCAGTAGTCCG3506990.14470502343580252No Hit
TAGTCAGACGTCAGTACGTACGAGTCCCTCCTTGTGTACTCGCAGTAGTC3398620.14023347279273313No Hit
ACCGAGACACAGTACGTACGAGTCACTCGAGTCGTACTCGCAGTAGTCCG3343520.13795994284502505No Hit
GGTTACGCACAGTACGTACGAGTCCTGACGGATGTACTCGCAGTAGTCCG3323720.1371429574917652No Hit
TAACTCCGAGTCAGTACGTACGAGTCTTAAGTGGTGTACTCGCAGTAGTC3234460.13345992150025116No Hit
CCACTGAAGTCAGTACGTACGAGTCGCACATGTTGTACTCGCAGTAGTCC3217630.13276548394998025No Hit
CCTCGGTCAAGTACGTACGAGTCCCAGGAGTTGTACTCGCAGTAGTCCGA3092040.12758340362089396No Hit
GAGAACAGGGTCAGTACGTACGAGTCCTCGACGATGTACTCGCAGTAGTC3067530.12657207478208587No Hit
ACTCGAGTCGTCAGTACGTACGAGTCCCACGTTCTGTACTCGCAGTAGTC3037090.12531606295616513No Hit
GCAGCTCTTAGTACGTACGAGTCTGACAAGTTGTACTCGCAGTAGTCCGA2974810.12274627266318401No Hit
ATCACTTCCCAGTACGTACGAGTCCTTCCTGATGTACTCGCAGTAGTCCG2810650.11597272137070205No Hit
ACATTCCACAGTACGTACGAGTCAGACCAGCTGTACTCGCAGTAGTCCGA2777340.11459828793044513No Hit
CCGCAATGGGTCAGTACGTACGAGTCCCGTCAGGTGTACTCGCAGTAGTC2750520.11349164413375673No Hit
ACCTTGAAGGTCAGTACGTACGAGTCAATCCTCGTGTACTCGCAGTAGTC2717290.11212051164442209No Hit
AATGCACTTCAGTACGTACGAGTCCTGACGGATGTACTCGCAGTAGTCCG2707020.1116967520697767No Hit
TCAGAGCGTAGTACGTACGAGTCCAATTGTCTGTACTCGCAGTAGTCCGA2706750.11168561136041405No Hit
GTCCTTCATCAGTACGTACGAGTCTCAAGGAGTGTACTCGCAGTAGTCCG2663880.10991671428494681No Hit
GGTCTTGCAGTCAGTACGTACGAGTCCGTTCCATTGTACTCGCAGTAGTC2506210.10341095263603337No Hit
AACGAGAATGTCAGTACGTACGAGTCTATACCACTGTACTCGCAGTAGTC2478670.1022746002810446No Hit
GCACATGTTCAGTACGTACGAGTCGACGGTGCTGTACTCGCAGTAGTCCG2474220.10209098488599379No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)