FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007772647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57104852
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGGGAATTGTTGAAGGACAGCATCAGCATGTAGCGTGCTAAAATAGC9571591.6761430359717944No Hit
TGGAGGAACATGCCGAGGAATTGAATTTCCAAGATGCAAAAATACTTGAA8220111.4394766315128529No Hit
CAATTTACTTACCCCAGCAAGACATTTTTCCAGTGTATCCAATATAATCA7726971.3531196963788648No Hit
GAAGACTTATCTCCTGCCTTTGATCATTCACCAAACAAAATAATGACTGA7525211.3177881977524433No Hit
AGCAAACCCAACATGAACTACGATAAGCTCAGCCGCGCCCTCCGTTACTA7505021.3142525962592462No Hit
TGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTGAAAGA7446601.3040222921863103No Hit
GCCATATAGATAAATCGGTACTGTGCTTCTGTCTGGACCATCCCTGACCT7033871.2317464722612363No Hit
GCTCTCTGTCAATCTCTCTGGTTGTGCTCAGAACTCCAGCAGTGCTCAGA6716651.1761960262150755No Hit
GCTTTGGGACTTGAGATTGTTTTTGTCTTTTTTCTGGCATTATTTCTGCT6664191.167009416292682No Hit
CCAAGTTTCCAGAAAACAGAAATCGAAACAGATACAGAGATGTAAGCCCA6081581.0649848107477802No Hit
GTCCCTGTCCGGCCAATTCCAGCACTGCGGGCAGAAGGACAAAAAGCAGG5665850.9921836414180707No Hit
TGTGACTTTGGATTGGCTCGAGATATCATGAGTGATTCCAACTATGTTGT5477940.9592775058763834No Hit
CCAAGCCAAAACGACTGCAGGAGTGGTACAAAAAGATGCTGGACAAGGCG5361490.9388851931531143No Hit
AACGTGCCCAACTTTACTCGGCTCAGGGGCTCGGACACCCCAGACATCCT5243010.9181373939993751No Hit
CCCGAATGACCCCCAGATTTGACTCCAACAACGTGGTCCTCATTGAGGAC5088120.8910136042380427No Hit
ACACACATGGCAAAGACAAAGAAAGGAAAGCGTCTCTCGACCAAGGGAAA5038350.8822980576151394No Hit
CATTCACACCAGAGTCATCATCCGTGGCTAAAATGGCTGCTACCAAAGAA5031760.8811440400896231No Hit
TGCTTGTAAGAGAGAGACCCAGGTCCTTGGAGGATCTCCATCAGAAAAAA4991690.8741271232083746No Hit
TCCACTCGGGCCACAAAGTTACTGGGCACATAGCCTTCTCTTCCTGTGAC4967300.8698560325486878No Hit
TTTACTTACTCTCGTCTCCACAGACACATACTCCATAATTTAAAACCAAA4967140.8698280139137738No Hit
TTGTAGTAGTGGCGCAGGGCTCTGGACATTTTCTCATAGGTCATGTTTGT4946490.8662118588451994No Hit
TGCTTGTAAGAGAGAGACCCAGATCCTTGGAGGATCTCCATCAGAAAAAA4897150.8575716123036271No Hit
TGGGACATCACCCTCACTTACTGGGTCACACTTGATCACTCTGGAGAGGA4882120.854939611786403No Hit
CTTGTGCCTTCTGCTTCTCCCCTGCTGTTGCAGAGAGCCCTGCTGACGAA4824570.8448616590408113No Hit
CCGGACAAAAGTACAGCAGCCACCCAACCAACCAGTCCCAGCTTCCAGTC4809010.8421368467954352No Hit
TCGTACAAATTTACTCACCTGCAAAGAGCCGATAGGTTCGGTGGCATTTA4698640.8228092421988942No Hit
CTGTTTGTTGGACATACTGGATACAGCTGGACAAGAAGAGTACAGTGCCA4664880.8168973102320622No Hit
TTTCTCTTTTGTTATTAGGAACAATCTCAGTTACAAAGTGAACTACTAAA4536030.7943335533029663No Hit
CGTGAAACTGCTTCAGATGCTGCTCCATGGTCTCAATGATGCTCTTTTGC4484870.7853745947892484No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATTGAGCAC4473070.7833082204643486No Hit
TTTCTCTTTTGTTATTAGGAACAATCTCAGCTACAAAGTGAACTACTAAA4361200.7637179411654897No Hit
GCGTGTAGTAAATAGAATCCCTCAGTAGGCACCAGGAGGAAAGAGCCACA4360420.7635813503202845No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCCGTGGCCCCTGCACCA4332210.7586413147520285No Hit
TCTCATCAAAGGAATGCACTAGGATTCGCGCGGGGGTCTTTCTGGTTCCT4321870.756830610470718No Hit
GGCTTCCGAATGGTAAACTGAAATGTGACGTCTGTGGCATGGTTTGCATT4206790.7366782073088991No Hit
AAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATTGATGCCAAAGA4088750.7160074594011732No Hit
GCATTCACCATAAATATCCAGGTTTGGTGTAGGCATAAGATACACGTTGA4064840.711820424646228No Hit
GTGGTCTCAGCTAATTTGCTTAGTTCTCGAACTCTGCTTTCTATTTCAGC4048650.7089852890258782No Hit
GCGTGTAGTAAATAGAATCCCTCAGCAGGCACCAGGAGGAAAGAGCCACA3970480.6952964347057585No Hit
AGGAATACCGGCCACGAACGTTCTGGTTTAACATGGCCGACGCCGCCTTC3920710.686580888082855No Hit
GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCG3918450.6861851248646963No Hit
CAGCAACTCTGTGCCATTCCAGAGATCGCTAACAGTGAGGAGGTGCAGGA3908740.6844847439583592No Hit
ATCATGGCCCGTCACCTCCACCCCATCCAGACCAGCTTCCAGGAGGCTAC3861890.6762805374226345No Hit
GGTTTCATTTTGCCAATTGAAGTTTATTTTAAAAACAAGGTATGTAATCT3684680.6452481480908138No Hit
CTAAAGAGGGAGCAACGCGAGGGTGGCTTACGGGTTGGTGGTGCTGAAGA3665000.6418018559964047No Hit
TTCCTGGAGCAGCATTTGAATTACTGTCCTGTGACATAGCACGGACTTCC3549880.6216424481758573No Hit
TGATATGGAGGATGCTGGTAACAGACGTGGTCCCATTTGGGTGAGACAGA3539680.6198562602000965No Hit
GTTCCATGTAATACTGGACCAACTCAGCCAAAGTGGCAAATTTCTCCCCT3474950.6085209712127438No Hit
AGAGACAGCGGGATTCCTTCCCACACCAGGATGAGAATCACAATCACTGC3461040.6060851011399171No Hit
CCGGCTGTCAGAAAGCAACTGATAGACGTAATCCCAAAGCAGTCTACAGT3331820.5834565511175828No Hit
TCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAATATTTCACCCTTCA3322010.5817386585644246No Hit
ATCTCCTTTGTCCCCGCCACCTGAGGAATTGCCCACTTCCCCGCCACCTG3297530.5774518074225987No Hit
TTGGAGAGCAGCAGCAGGTTCTCCACGGCGCTGTCCTGGGCCGAGTGGTT3277450.573935468740905No Hit
GTGGGTATTAAACAAGAACCAGATGACGGGAGCTATTACCCAGAGGCCAG3253400.5697239176804101No Hit
CCCATTTCTTCTCCTTACTGCAGGTGAACGCCCCTTCCACTGTAACCAGT3243280.5679517390221063No Hit
GCTATCCCAACAGATTGCCAAGTATGCCCACGGACATGGAACACACAGGA3241200.5675874967682255No Hit
CCATGATGACCACCAGTCCTACCAAGGACACCCTGCCTTAAATCCCAAGA3232330.5660342136951866No Hit
GGTGTTTGTCTCCTCTTCATTGTCGTTTTAACCCTGCTAATTTGTCACAA3231930.5659641671079018No Hit
GGAACACCAGGCAGATTCATTTACACAGGTAAGAACCACAGCACATGGTG3060700.535978974255988No Hit
TGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGG3058210.5355429342501404No Hit
GCTATCCCAACAGATTGCCAAGCATGCCCACGGACATGGAACACACAGGA3044370.5331193223300885No Hit
AGCAAGAGAAACTGGGCACAGTTTACTGTCAGGCAAGCTTTCCTGGAGCA3036940.5318182069712746No Hit
GCTCCCCCTACTATTATAGCGCTGCCGCCCGAGGAGCCGCCCCACCTGCA3024420.5296257487892623No Hit
TCGTGGTTCACATCCCGCGGCTCACGGGGGAGTGGGCAGCGCCAGGGGCG2962860.5188455790061411No Hit
ATTATGAGACAGGAAGCATCAAGCCTGGGGTAATTGGAGGATCCAAACCA2868110.502253293643069No Hit
AGCCTGTCTGAAGTGCAGTCTCTGAGCTCCTTCCAGTCCGAATCGTGCGA2806980.4915484239412791No Hit
TGTACACACCGATGTCATGGTCGTTCCTCAGGGTGCAGGTGAGGCGGATT2740030.47982437639449615No Hit
TGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCCT2701590.47309289935643295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2661960.4661530337211976No Hit
GCTTCTTACCAGCATTCTGTGTGCTCCTGATGCAGCGAAGGCGTATCCCT2606210.4563902906183874No Hit
GTCACTCTTGGAGCTTTGCTGTCCTCCCGAGGTGGTGGAGAGGTCCTCGG2601910.45563728980507645No Hit
TGGCTCTGACTGTACCACCATCCACTACAACTACATGTATAACAGTTCCT2390460.41860891260168226No Hit
CCCATCTGCCCAAGAGACTCCGTCAAAGTGATCAACAAGGAAGGGGAGGT2368910.414835152711717No Hit
TTCTCCTTCCACCAGTTCCACGTTGCGCATCTCCTCCATGTAGCAGGCAT2356370.4126391922003405No Hit
TGCAGTACACCGGATTGGCCGCACCGGGCGCTCGGGAAACACAGGCATCG2324740.40710025831079993No Hit
TTCTCCTTCCACCAGTTCCACATTGCGCATCTCCTCCATGTAGCAGGCAT2314050.4052282632656153No Hit
GAAAGACGTCACACTGAGAGTATCTGGAGACCTTCATGTTGGAGGAGTGA2309940.4045085345812646No Hit
CGGTCTGAAGAGCCAAGAGCTATTTGATGAAATTAGAATGACCTACATCA2207020.3864855476729018No Hit
CTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGATGT2178130.3814264329062616No Hit
GGGATAGAGGAGCGGATGACGGTCGTCTGGGACAAGGCGGTGGTAAGGCT2148720.37627625757615135No Hit
GTGGCAGCGCTCTTTATGTTCCTCTAAAGAGCTTCGCTGTTTATAGCTTC2131380.373239738017358No Hit
TACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT2126550.3723939254758948No Hit
GGAAGCCACGAGAATATTGTGAACCTGCTGGGGGCGTGCACACTGTCAGG2115440.3704483815140612No Hit
AGCTTATTGAGTTCATCATTAAAGTCATCTATTTTCAACCTGGGGTACAA1969900.34496193073050957No Hit
GCTTTCAAAAGATTACATCAAATATGGACTCTGTGGGTCGAATACAAATG1945460.3406820842474121No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCA1936740.3391550686446049No Hit
ACAGTTCTCTGAATGCCAATATGATTATTTCCAGATTAAAATGCAAAATT1920200.33625864226038094No Hit
ACAGTTCTCTGAATGCCAATATGATTACTTCCAGATTAAAATGCAAAATT1861780.3260283381874451No Hit
GTGAGTGGATGGGTAAAACCTATCATCATAGGTCGTCATGCTTATGGGGA1844740.32304435356911526No Hit
AGCTTATTGAGTTCATCATTAAAGTCATCGATTTTCAACCTGGGGTACAA1839400.322109231628864No Hit
CTTTAGCATTTACTAACATTCCAGATGTAAAAAGTGCCTCAGAACGACCC1802900.3157174805391318No Hit
CTAGGTGGTGCTGAGAGAGGTGTGGGGTGGCTGAGTGGTCCAGAAGCAGC1784460.31248833286530536No Hit
TCGTACAAATTTACTCACCTGCAAACAGCCGATAGGTTCGGTGGCATTTA1713440.30005156129290034No Hit
AGGACCATTGCCTCAAGGGCATGAATTTGAACTGTACGAAGTGAGATTTC1681590.2944741017803531No Hit
GTCACAGTCAGCAGTAGCATATGCATGAGGTACAGTACTTTTAGATTCAG1681450.29444958547480343No Hit
CATCACCAGGTGCGTTACCTGTTGGAATGGACAGAGGAGGACCTGGAGGA1614130.28266074483478215No Hit
AGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCG1600050.28019510496235944No Hit
GCTTTCAAAAGATTACATCAAATATGGACTCCGTGGGTCGAATACAAATG1574820.27577691646937463No Hit
ATGGTGTGGTTGACCTGGCCCAGCCCCTCGCTGCTCTCCGAGTGGGTGCA1539610.2696110656236356No Hit
GCTCATGACTGCTAAGCTGAGCGCCTTGCTTGTGGCTGGAGCCTGGATGG1511280.2646500160791941No Hit
CAACATCTCCTTCGCAGCCGCGCAAAAAAAGTAGGCCATTTATTCATTAC1505850.2636991336568038No Hit
TCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAGCCAAGCCGAGT1500450.2627535047284598No Hit
TCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAACCAAGCCGAGT1498670.2624417974150428No Hit
TGTCCAGCACGAGAGATCTCTCTGATCCTATCTTGCACAGGTCTTCTGCC1434880.2512711179078093No Hit
CTCACTCACCACTGCAGCTGGCTTTGGAGGCAGCTCAGAGTTAGAGGTCT1239880.21712340660649992No Hit
AGTAACTTGAGGGGCACGACCGAGGGGGTTTACTACCAGGCCCCCGACTT1237820.21676266668198352No Hit
TCTCGAGAACTCTGTGTGCCACTGGGAGTGCCTCTAGACCGTTCATATCC1234030.21609897526746066No Hit
GGAACACCAGGCAGATTCATTTATACAGGTAAGAACCACAGCACATGGTG1200380.2102063061121321No Hit
TCCACCTGCCTTCAGAAGCCCAGGAGGTGGCCCTTGTGGCCTATAACTCA1192280.2087878627196162No Hit
AGATGTACCTATGGTCCTAGTAGGAAATAAATGTGATTTGCCTTCTAGAA1057460.18517866047529552No Hit
GATTCACCAGGTGCTCATGGACCATGGAGTCCCATGTCATTACACCTGCT1039290.1819967942478863No Hit
GATTCACCAGGTGCTCATGGACCGGGAGGACACGTGTCACCGTACCTGCT1026240.17971152433772178No Hit
GTCTCTCTCTCTCTCTTTAGGCCAAGGCAGGTGGATCACCTGAGGTCAGG992970.17388539944031375No Hit
AGGTTAAGGTTGGCAGTGATACTAAGCTGGCTCTGCAGTGCAGGAGGGTA978680.1713829851095665No Hit
GTGCTCATGTGCGGAAACAACAACTGCTGCAGGTGAGGCTGTTGTGGCCT946150.16568644639863528No Hit
ATTTGCCAACAAGGACAGTTGATACAAAACAAGCCCACGAACTGGCCAAG871700.15264902534026356No Hit
ACAATGAGCTTTCACCCGAAAGGGAGCACTCCAATATGGCAATTGACCTC854930.14971232216835093No Hit
AACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACAATC783570.13721601099675382No Hit
CGTTATGTGCGACGAGAACTCGTACCGGTCCTGGCTGTGGTAGCCGCACA767840.13446142895178154No Hit
GGATCCCAACGGAGTCAACCGTTTCGGGAGGCGCGCGATCCAGGTAGCTG766980.13431082878911935No Hit
AGGTGATCAGTTGGTCCTTCTCGGTCCTTTGATTGTTTAATAAGATCAAA755010.1322146846646236No Hit
CAGGAAATTACACACTGCCATGATGAAGATGATGATGAAGAGGAGGAGGA710160.12436071106532243No Hit
CAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGA700500.12266908598239604No Hit
AGGATAATCTTGCTGGAAAACTTCGAAAGCGGGGGTCGCGGAACCACTGG654260.1145717004922804No Hit
GCACCTTGCAGGCACCTTTGGGGGGCCGCGGGGGTCTGTAGTAGAACTCG622840.1090695410610643No Hit
TGACCATCAATGTGGGGCGTGCTGGGGCTGCCAGCCTGGATGTCATCCAG580010.10156930272755106No Hit
GCTTCACTGCCGTTCTTGGCCATTCCCTAGGAATAGAGGAAAAGAACAAG573410.1004135340373529No Hit
TAAGCTTGCCTTGGGAGCCCCCCAGGCATGTTGGAATTTCCAGTGTATTG571380.10005804760688287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)