FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007772648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253417976
Sequences flagged as poor quality0
Sequence length139-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACCGTGTCAGTACGTACGAGTCGTACGCGATGTACTCGCAGTAGTC10072200.3974540464327598No Hit
AACGCATTGGTCAGTACGTACGAGTCCTCCGCATTGTACTCGCAGTAGTC9695460.38258769772512113No Hit
GCATTGATCCAGTACGTACGAGTCTAGCCTTGTGTACTCGCAGTAGTCCG9386070.37037901368133413No Hit
GTAGAGAGCGTCAGTACGTACGAGTCCTGTCACTTGTACTCGCAGTAGTC8864580.3498007576226558No Hit
ATTAGCGGTGTCAGTACGTACGAGTCGATAGTTCTGTACTCGCAGTAGTC8034610.3170497265750398No Hit
CTCAGGTACTCAGTACGTACGAGTCGAGTTAACTGTACTCGCAGTAGTCC7778100.3069277137624996No Hit
ATTGATCACGTCAGTACGTACGAGTCCGACGTGATGTACTCGCAGTAGTC7707350.3041358833992108No Hit
GAACCAGTTCAGTACGTACGAGTCCTTCTGAGTGTACTCGCAGTAGTCCG7087350.2796703735018387No Hit
AGAATCAGAAGTACGTACGAGTCCTGACGGATGTACTCGCAGTAGTCCGA7033480.27754463637575577No Hit
TCATCTGTCCAGTACGTACGAGTCGACTCAGTGGTACTCGCAGTAGTCCG6962490.2747433354925066No Hit
GTGGAGGTCCAGTACGTACGAGTCTCGAACGCTGTACTCGCAGTAGTCCG6922670.273172018389098No Hit
ATCCACAGTTCAGTACGTACGAGTCATGCGGATTGTACTCGCAGTAGTCC6901600.2723405856575857No Hit
ACAAGAATGGTCAGTACGTACGAGTCCAGTAATGTGTACTCGCAGTAGTC6736680.26583276002488476No Hit
GGAGATGAGTCAGTACGTACGAGTCTATACGGATGTACTCGCAGTAGTCC6640940.2620548117707325No Hit
GAGCCGTTACAGTACGTACGAGTCTGTCTACACGTACTCGCAGTAGTCCG6538440.2580101105376992No Hit
TCACTACGTGTCAGTACGTACGAGTCGAGATTCCTGTACTCGCAGTAGTC6423610.2534788613417069No Hit
GTGTACCGTGTCAGTACGTACGAGTCCAAGCGCATGTACTCGCAGTAGTC6369610.2513479943506454No Hit
AACGAGGTGTCAGTACGTACGAGTCGGTTGCTATGTACTCGCAGTAGTCC6334770.24997319053641245No Hit
CTATCCATCGTCAGTACGTACGAGTCGGTAAGGATGTACTCGCAGTAGTC6241450.2462907366918596No Hit
TATTCAACGTCAGTACGTACGAGTCGGTCGGTCTGTACTCGCAGTAGTCC6109950.24110168096362666No Hit
ATATGCAGCTCAGTACGTACGAGTCCCTCTGCATGTACTCGCAGTAGTCC5871730.23170140069305897No Hit
CTTAGGACTGTCAGTACGTACGAGTCGGTTGCTATGTACTCGCAGTAGTC5843830.23060045274767724No Hit
TCTAGGATGCAGTACGTACGAGTCCTCTGCGGTGTACTCGCAGTAGTCCG5707760.22523106253520075No Hit
ATCTTACAGTCAGTACGTACGAGTCACTACTCATGTACTCGCAGTAGTCC5682340.2242279766294085No Hit
TTCGCTGAGCAGTACGTACGAGTCCCTGCAGATGTACTCGCAGTAGTCCG5549000.21896631358148008No Hit
ATGTGAGACGTCAGTACGTACGAGTCATCTCTGCTGTACTCGCAGTAGTC5423110.21399863125731855No Hit
GCCGTCTTCAGTACGTACGAGTCTGGAAGATTGTACTCGCAGTAGTCCGA5327320.21021870997817454No Hit
TTCACTGGAGTCAGTACGTACGAGTCGCCATCGCTGTACTCGCAGTAGTC5249160.20713447731111229No Hit
TTCATCCAATCAGTACGTACGAGTCCGGTAGCTTGTACTCGCAGTAGTCC5179620.20439039415262317No Hit
TTAAGTGGTGTCAGTACGTACGAGTCCGACCACTTGTACTCGCAGTAGTC5158700.20356488049608604No Hit
TTGTATCACCAGTACGTACGAGTCCGGAGACTTGTACTCGCAGTAGTCCG4995980.1971438679630209No Hit
TGGACTGCCGTCAGTACGTACGAGTCTCTACATCCGTACTCGCAGTAGTC4959140.195690143149119No Hit
CCTAGTTAGAGTACGTACGAGTCGTTATCGATGTACTCGCAGTAGTCCGA4886610.1928280730961248No Hit
TGCGTATCTGTCAGTACGTACGAGTCCTCCGCATTGTACTCGCAGTAGTC4676250.18452716235094546No Hit
TATCTGTCAAGTACGTACGAGTCTGCCGAACTGTACTCGCAGTAGTCCGA4461000.17603328976157553No Hit
TTAACGGAGAGTACGTACGAGTCGCCTTATCTGTACTCGCAGTAGTCCGA4269170.16846358207832898No Hit
TGTCTACACTCAGTACGTACGAGTCCCTCCTTGTGTACTCGCAGTAGTCC4252670.1678124838310602No Hit
ATCGTAGTGAGTACGTACGAGTCGGTGAGCGTGTACTCGCAGTAGTCCGA4148930.16371885157823216No Hit
CCTACTAGATCAGTACGTACGAGTCTGCAGATGCGTACTCGCAGTAGTCC4136170.16321533560034432No Hit
TTCAACTACTCAGTACGTACGAGTCTCTCAGGATGTACTCGCAGTAGTCC4088620.1613389888332152No Hit
CAGTAATGTCAGTACGTACGAGTCACACACCGTGTACTCGCAGTAGTCCG4074400.16077786052556903No Hit
ACGGTGTACGTCAGTACGTACGAGTCAAGACTGGTGTACTCGCAGTAGTC4073970.1607608925106402No Hit
GGTAATCTAGTCAGTACGTACGAGTCGATCTACTTGTACTCGCAGTAGTC3958560.1562067562247439No Hit
AGTAATCAGAGTACGTACGAGTCACACACCGTGTACTCGCAGTAGTCCGA3934720.1552660179086901No Hit
TTCGCTGAGCAGTACGTACGAGTCCTTCACTGTGTACTCGCAGTAGTCCG3901610.15395948075917076No Hit
CATGCCAGTAGTACGTACGAGTCATAGTCAGTGTACTCGCAGTAGTCCGA3881600.153169876157483No Hit
GTAAGTGAGGTCAGTACGTACGAGTCTAAGGTGATGTACTCGCAGTAGTC3823460.15087564269710685No Hit
GTATCGTCGTCAGTACGTACGAGTCCGCATAACTGTACTCGCAGTAGTCC3748730.14792675954447682No Hit
AGGTATTCCTCAGTACGTACGAGTCATTGATCACGTACTCGCAGTAGTCC3682710.14532157734540505No Hit
CGCTTGTAGGTCAGTACGTACGAGTCTTCCAGATTGTACTCGCAGTAGTC3670340.14483345096245262No Hit
GACTCTCACAGTACGTACGAGTCCAACACTCCGTACTCGCAGTAGTCCGA3573250.14100223103352383No Hit
TCTGAGATACAGTACGTACGAGTCCATTCAGTTGTACTCGCAGTAGTCCG3549380.140060308902475No Hit
CCACAAGGTTCAGTACGTACGAGTCATCCTGCCTGTACTCGCAGTAGTCC3537390.1395871775094597No Hit
GACTTGATTCAGTACGTACGAGTCCGCACTCTTGTACTCGCAGTAGTCCG3485090.13752339336811686No Hit
CAGTCGGTATCAGTACGTACGAGTCTAATCACGCGTACTCGCAGTAGTCC3343810.13194841395150278No Hit
TCACGCGACTCAGTACGTACGAGTCTATACCACTGTACTCGCAGTAGTCC3170370.1251043848602121No Hit
GCGGTAAGTTCAGTACGTACGAGTCTATGTACCTGTACTCGCAGTAGTCC3121630.12318108009828001No Hit
CCATAAGTAGTCAGTACGTACGAGTCTAGTAGTCTGTACTCGCAGTAGTC3106800.1225958808857348No Hit
TAGATCTGTTCAGTACGTACGAGTCCTGGACACTGTACTCGCAGTAGTCC3106340.12257772905581094No Hit
ATCGTAGTGAGTACGTACGAGTCCGTGTCCGAGTACTCGCAGTAGTCCGA3073260.12127237572128664No Hit
GCTACCTTAAGTACGTACGAGTCATCCTGCCTGTACTCGCAGTAGTCCGA3067870.12105968362717884No Hit
CCGATCAGCCAGTACGTACGAGTCCACAACGCTGTACTCGCAGTAGTCCG3017970.11909060468543874No Hit
AGACGCTATTCAGTACGTACGAGTCAAGTTCTCTGTACTCGCAGTAGTCC3007650.11868337232714699No Hit
TCCACCTCACAGTACGTACGAGTCCTGACGGATGTACTCGCAGTAGTCCG2990100.11799084055505202No Hit
TGTGTCTACGTCAGTACGTACGAGTCAATATCGCTGTACTCGCAGTAGTC2769890.10930124388650313No Hit
CTATAGCTCTCAGTACGTACGAGTCCGTTCCATTGTACTCGCAGTAGTCC2751370.10857043542956875No Hit
TGGAATCGTTCAGTACGTACGAGTCCAGTTGAGTGTACTCGCAGTAGTCC2736600.1079876038470136No Hit
GATACACTAAGTACGTACGAGTCACAACGAGCGTACTCGCAGTAGTCCGA2729850.10772124547313092No Hit
GCGAAGCTCGTCAGTACGTACGAGTCGTAGTTGGTGTACTCGCAGTAGTC2648600.10451507986158014No Hit
ATGGTTCGAGTCAGTACGTACGAGTCGACACCGATGTACTCGCAGTAGTC2637880.1040920633033546No Hit
GCTTCTACGTCAGTACGTACGAGTCCCAGTCTCAGTACTCGCAGTAGTCC2627760.10369272304502977No Hit
ACTCTGCTCAGTACGTACGAGTCCCTCGCGTTGTACTCGCAGTAGTCCGA2626850.1036568139901804No Hit
GCATACATGAGTACGTACGAGTCTTGCCAGATGTACTCGCAGTAGTCCGA2622220.1034741118759468No Hit
CCGACTGTTGTCAGTACGTACGAGTCGACTCTGGTGTACTCGCAGTAGTC2615850.1032227484920012No Hit
TGCTGCGTCTCAGTACGTACGAGTCTCAGAGCGTGTACTCGCAGTAGTCC2598980.10255704985979369No Hit
TGTCACATAGTCAGTACGTACGAGTCTGACCAAGTGTACTCGCAGTAGTC2598060.10252074619994597No Hit
CACAACGCTGTCAGTACGTACGAGTCGGTAAGGATGTACTCGCAGTAGTC2582060.10188937820259443No Hit
ATCATGTTCGTCAGTACGTACGAGTCGGACTCCTTGTACTCGCAGTAGTC2576320.10166287493354458No Hit
CTTCACTGTAGTACGTACGAGTCCCTGCAGATGTACTCGCAGTAGTCCGA2574470.10158987300885079No Hit
AAGTCATGAGTCAGTACGTACGAGTCCCTCGGTCAGTACTCGCAGTAGTC2574460.10158947840385245No Hit
CGCACTCTTTCAGTACGTACGAGTCGGTAAGGATGTACTCGCAGTAGTCC2557530.10092141214165486No Hit
TGATTGTGCCAGTACGTACGAGTCTGTAACAGTGTACTCGCAGTAGTCCG2547520.10052641253831181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)