FastQCFastQC Report
Fri 6 Jan 2023
EGAF00007772651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110521797
Sequences flagged as poor quality0
Sequence length130-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGTAAGAGAGAGACCCAGGTCCTTGGAGGATCTCCATCAGAAAAAA29555992.6742227146379096No Hit
AGCAAACCCAACATGAACTACGATAAGCTCAGCCGCGCCCTCCGTTACTA28696122.5964217718971763No Hit
TTTCTCTTTTGTTATTAGGAACAATCTCAGTTACAAAGTGAACTACTAAA22177732.0066385637938913No Hit
GAAGACTTATCTCCTGCCTTTGATCATTCACCAAACAAAATAATGACTGA19742841.7863299852064478No Hit
GCCATATAGATAAATCGGTACTGTGCTTCTGTCTGGACCATCCCTGACCT18846521.7052310504868102No Hit
TGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTGAAAGA18003871.628988171446398No Hit
TCGTACAAATTTACTCACCTGCAAACAGCCGATAGGTTCGGTGGCATTTA16125851.459065129026087No Hit
TGTGACTTTGGATTGGCTCGAGATATCATGAGTGATTCCAACTATGTTGT15865481.4355068801496234No Hit
GTCCCTGTCCGGCCAATTCCAGCACTGCGGGCAGAAGGACAAAAAGCAGG15743791.4244963823742387No Hit
CCAAGTTTCCAGAAAACAGAAATCGAAACAGATACAGAGATGTAAGCCCA15617821.4130986306710158No Hit
GCTTTGGGACTTGAGATTGTTTTTGTCTTTTTTCTGGCATTATTTCTGCT15515641.4038533955433243No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCCGTGGCCCCTGCACCA14803271.3393982365306638No Hit
AACGTGCCCAACTTTACTCGGCTCAGGGGCTCGGACACCCCAGACATCCT13864021.2544150001469845No Hit
GGCTTCCGAATGGTAAACTGAAATGTGACGTCTGTGGCATGGTTTGCATT12645341.1441489681895056No Hit
ATCTCCTTTGTCCCCGCCACCTGAGGAATTGCCCACTTCCCCGCCACCTG12196271.1035171641300765No Hit
GCTCTCTGTCAATCTCTCTGGTTGTGCTCAGAACTCCAGCAGTGCTCAGA11713051.0597954718380123No Hit
TTGTAGTAGTGGCGCAGGGCTCTGGACATTTTCTCATAGGTCATGTTTGT11605591.0500725028928004No Hit
GCGTGTAGTAAATAGAATCCCTCAGTAGGCACCAGGAGGAAAGAGCCACA11541561.0442790755564715No Hit
GGTGTTTGTCTCCTCTTCATTGTCGTTTTAACCCTGCTAATTTGTCACAA11230541.0161380202676218No Hit
GCGTGTAGTAAATAGAATCCCTCAGCAGGCACCAGGAGGAAAGAGCCACA11028650.997871035339753No Hit
GTTCCATGTAATACTGGACCAACTCAGCCAAAGTGGCAAATTTCTCCCCT10579840.9572627560516411No Hit
GCTATCCCAACAGATTGCCAAGCATGCCCACGGACATGGAACACACAGGA10473800.9476682685497775No Hit
TCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAATATTTCACCCTTCA10441330.9447303865318079No Hit
TCCACTCGGGCCACAAAGTTGCTGGGCACATAGCCTTCTCTTCCTGTGAC10419070.9427163041874899No Hit
GTGGTCTCAGCTAATTTGCTTAGTTCTCGAACTCTGCTTTCTATTTCAGC10308670.9327273243666134No Hit
CGGTCTGAAGAGCCAAGAGCTATTTGATGAAATTAGAATGACCTACATCA10157090.9190123826886384No Hit
CAATTTACTTACCCCAGCAAGACATTTTTCCAGTGTATCCAATATAATCA9943810.8997148318172931No Hit
CTAAAGAGGGAGCAACGCGAGGGTGGCTTACGGGTTGGTGGTGCTGAAGA9692400.8769672827523787No Hit
CATTCACACCAGAGTCATCATCCGTGGCTAAAATGGCTGCTACCAAAGAA9395430.8500974699135593No Hit
GCTTTCAAAAGATTACATCAAATATGGACTCTGTGGGTCGAATACAAATG9393270.8499020333518464No Hit
TCTCATCAAAGGAATGCACTAGGATTTGCGCGGGGGTCTTTCTGGTTCCT9266130.8383984201776958No Hit
TGGAGGAACATGCCGAGGAATTGAATTTCCAAGATGCAAAAATACTTGAA9248290.8367842589457716No Hit
TGGAGGAACATGCCGAGGAACTGAATTTCCAAGATGCAAAAATACTTGAA9064890.82019024717812No Hit
CCATGATGACCACCAGTCCTACCAAGGACACCCTGCCTTAAATCCCAAGA9018980.8160363154428262No Hit
AAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATTGATGCCAAAGA8929590.8079483181041655No Hit
TGTACACACCGATGTCATGGTCGTTCCTCAGGGTGCAGGTGAGGCGGATT8839680.7998132712228702No Hit
CAGCAACTCTGTGCCATTCCAGAGATCGCTAACAGTGAGGAGGTGCAGGA8710220.7880997447046577No Hit
TCAAGGGAATTGTTGAAGGACAGCATCAGCATGTAGCGTGCTAAAATAGC8618850.7798325971844269No Hit
AGGAATACCGGCCACGAACGTTCTGGTTTAACATGGCTGACGCCACCTTC8271910.7484415042582053No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATCGAGCAC7847000.7099956943334897No Hit
GGAACACCAGGCAGATTCATTTATACAGGTAAGAACCACAGCACATGGTG7741900.7004862579279271No Hit
GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCG7640250.6912889771417668No Hit
AGAGACAGCGGGATTCCTTCCCACACCAGGATGAGAATCACAATCACTGC7573870.6852829220646856No Hit
TGGGACATCACCCTCACTTACTGGGTCACACTTGATCACTCTGGAGAGGA7380250.6677642058244855No Hit
ATCATGGCCCGTCACCTCCACCCCATCCAGACCAGCTTCCAGGAGGCTAC7353960.6653854895247496No Hit
CCGGACAAAAGTACAGCAGCCACCCAACCAACCAGTCCCAGCTTCCAGTC7189070.650466260515109No Hit
CCCGAATGACCCCCAGATTCGACTCCAACAACGTGGTCCTCATTGAGGAC7058970.6386948268675001No Hit
TTTACTTACTCTCGTCTCCACAGACACATACTCCATAATTTAAAACCAAA6497350.5878795112243786No Hit
CTTGTGCCTTCTGCTTCTCCCCTGCTGTTGCAGAGAGCCCCGCTGACGAA6464740.5849289620218535No Hit
CTGTTTGTTGGACATACTGGATACAGCTGGACAAGAAGAGTACAGTGCCA6213580.5622040329293596No Hit
TTCTCCTTCCACCAGTTCCACGTTGCGCATCTCCTCCATGTAGCAGGCAT6192320.5602804304747234No Hit
TTCTCCTTCCACCAGTTCCACATTGCGCATCTCCTCCATGTAGCAGGCAT6182310.5593747267790081No Hit
CCAAGCCAAAACGACTGCAGGAGTGGTACAAAAAGATGCTGGACAAGGCG6116930.5534591515916087No Hit
CGTGAAACTGCTTCAGATGCTGCTCCATGGTCTCAATGATGCTCTTTTGC6095280.5515002619799966No Hit
GGAAGCCACGAGAATATTGTGAACCTGCTGGGGGCGTGCACACTGTCAGG6092010.551204392740737No Hit
CAGCAACTCTGTGCCATTCCGGAGATCGCTAACAGTGAGGAGGTGCAGGA6021130.5447911781600873No Hit
TGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGG5991740.5421319742023377No Hit
ACAGTTCTCTGAATGCCAATATGATTATTTCCAGATTAAAATGCAAAATT5990500.5420197791391321No Hit
TGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCCT5938510.5373157296745726No Hit
ACACACATGGCAAAGACAAAGAAAGGAAAGCGTCTCTCGACCAAGGGAAA5516350.4991187394464822No Hit
GGAACACCAGGCAGATTCATTTACACAGGTAAGAACCACAGCACATGGTG5509620.49850980978892334No Hit
GGTTTCATTTTGCCAATTGAAGTTTATTTTAAAAACAAGGTATGTAATCT5471410.4950525732041798No Hit
AGCTTATTGAGTTCATCATTAAAGTCATCTATTTTCAACCTGGGGTACAA5449100.4930339668653777No Hit
GTGAGTGGATGGGTAAAACCTATCATCATAGGTCGTCATGCTTATGGGGA5397710.4883842053346274No Hit
AGGAATACCGGCCACGAACGTTCTGGTTTAACATGGCCGACGCCGCCTTC5340060.4831680396944686No Hit
TGATATGGAGGATGCTGGTAACAGACGTGGTCCCATTTGGGTGAGACAGA5308260.48029077920258567No Hit
GCTCCCCCTACTATTATAGCGCTGCCGCCCGAGGAGCCGCCCCACCTGCA5301650.4796927071317887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4623230.4183093403738269No Hit
GCTTCTTACCAGCATTCTGTGTGCTCCTGATGCAGCGAAGGCGTATCCCT4535170.410341681288443No Hit
CCCATTTCTTCTCCTTACTGCAGGTGAACGCCCCTTCCACTGTAACCAGT4435050.4012828347334961No Hit
GTGGCAGCGCTCTTTATGTTCCTCTAAAGAGCTTCGCTGTTTATAGCTTC4288260.3880012917270971No Hit
GTCACTCTTGGAGCTTTGCTGTCCTCCCGAGGTGGTGGAGAGGTCCTCGG4213480.38123520557668816No Hit
TCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAACCAAGCCGAGT4121690.37293005650279104No Hit
TCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAGCCAAGCCGAGT4049370.36638655088099953No Hit
GCATTCACCATAAATATCCAGGTTTGGCGTAGGCATAAGATACACGTTGA4048180.36627887981227814No Hit
AGTAACTTGAGGGGCACGACTGAGGGGGTTTACTACCAGGCCCCCGACTT4025450.3642222719198096No Hit
CCGGCTGTCAGAAAGCAACTGATAGACGTAATCCCAAAGCAGTCTACAGT3918330.3545300661370897No Hit
GCATTCACCATAAATATCCAGGTTTGGTGTAGGCATAAGATACACGTTGA3842250.3476463561300944No Hit
GCTCATGACTGCTAAGCTGAGCGCCTTGCTTGTGGCTGGAGCCTGGATGG3777940.34182759442465455No Hit
ATTATGAGACAGGAAGCATCAAGCCTGGGGTAATTGGAGGATCCAAACCA3724890.3370276362770323No Hit
TACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT3678850.33286194215607984No Hit
GTGGGTATTAAACAAGAACCAGATGACGGGAGCTATTACCCAGAGGCCAG3583730.32425549504954215No Hit
CTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGATGT3567040.322745385690752No Hit
AGCCTGTCTGAAGTGCAGTCTCTGAGCTCCTTCCAGTCCGAATCGTGCGA3557950.321922923493544No Hit
TTCCTGGAGCAGCATTTGAATTACTGTCCTGTGACATAGCACGGACTTCC3528420.3192510523512389No Hit
GAAAGACGTCACACTGAGAGTATCTGGAGACCTTCATGTTGGAGGAGTGA3480570.3149215896299623No Hit
CTTTAGCATTTACTAACATTCCAGATGTAAAAAGTGCCTCAGAACGACCC3314490.29989468955160037No Hit
CAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGA3288470.2975404028220786No Hit
AGCAAGAGAAACTGGGCACAGTTTACTGTCAGGCAAGCTTTCCTGGAGCA3212930.29070555195551157No Hit
CCCATCTGCCCAAGAGACTCCGTCAAAGTGATCAACAAGGAAGGGGAGGT3192410.28884890461923995No Hit
ACATCCGAGTTAGAACTGAGGTTTGTTAATCTGGGGTCCGCATTGATTTC3133940.28355854546954207No Hit
TGCAGTACACCGGATTGGCCGCACCGGGCGCTCGGGAAACACAGGCATCG3103840.2808351007901183No Hit
AGGACCATTGCCTCAAGGGCATGAGTTTGAACTGTACGAAGTGAGATTTC2816160.25480584612644325No Hit
GTCACAGTCAGCAGTAGCATATGCATGAGGTACAGTACTTTTAGATTCAG2668200.24141844164911652No Hit
AGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCG2560290.2316547567535479No Hit
CATCACCAGGTGCGTTACCTGTTGGAATGGACAGAGGAGGACCTGGAGGA2374640.21485716523411214No Hit
TTCCTGGAGCAGCATTTGAATTGCTGTCCTGTGACATAGCACGGACTTCC2361770.21369268905390673No Hit
AGATGTACCTATGGTCCTAGTAGGAAATAAATGTGATTTGCCTTCTAGAA2345710.21223958202561616No Hit
ACAATGAGCTTTCACCCGAAAGGGAACACTCCAATATGGCAATTGACCTC2066300.18695859604961002No Hit
ACAGTGGATCTCTGTGCCACATGGGAGGTGAGTGTTTGGAGGTAAGAGAA2062880.1866491548268981No Hit
CTCACTCACCACTGCAGCTGGCTTTGGAGGCAGCTCAGAGTTAGAGGTCT2017400.18253412944416747No Hit
TCTCGAGAACTCTGTGTGCCACTGGGAGTGCCTCTAGACCGTTCATATCC1971780.1784064368768814No Hit
TGTCCAGCACGAGAGATCTCTCTGATCCTATCTTGCACAGGTCTTCTGCC1866320.16886442771103333No Hit
TTGGAGAGCAGCAGCAGGTTCTCCACGGCGCTGTCCTGGGCCGAGTGGTT1799770.1628429910527061No Hit
GATTCACCAGGTGCTCATGGACCGGGAGGACACGTGTCACCGTACCTGCT1646260.14895342318764507No Hit
AGGTGATCAGTTGGTCCTTCTCGGTCCTTTGATTGTTTAATAAGATCAAA1578760.14284603063412007No Hit
TGGCAAGCTGAGTCTCCCTAAGTGGACTCAGAGAGGGGGTGGTGAGGACT1577670.14274740755436685No Hit
GATTCACCAGGTGCTCATGGACCATGGAGTCCCATGTCATTACACCTGCT1565850.14167793525832736No Hit
CAACATCTCCTTCGCAGCCGCGCAAAAAAAGTAGGCCATTTATTCATTAC1512230.1368264035735865No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCA1460020.132102448533297No Hit
TCCACCTGCCTTCAGAAGCCCAGGAGGTGGCCCTTGTGGCCTATAACTCA1206470.10916127250446353No Hit
CTAGGTGGTGCTGAGAGAGGCGTGGGGTGGCTGAGTGGTCCAGAAGCAGC1181080.10686398810544132No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)