FastQCFastQC Report
Fri 2 Jun 2023
EGAF00007772652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232413571
Sequences flagged as poor quality0
Sequence length138-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCCGCATCAGTACGTACGAGTCGTAGTTGGTGTACTCGCAGTAGTCC50219032.1607615159443507No Hit
GGTCCTAAGAGTACGTACGAGTCATGATAGCTGTACTCGCAGTAGTCCGA25930441.115702490540021No Hit
GAATCAACCCAGTACGTACGAGTCCACCTTCCTGTACTCGCAGTAGTCCG24634611.0599471405221856No Hit
AGTATGACGAGTACGTACGAGTCCCATATCTTGTACTCGCAGTAGTCCGA23094420.9936777745220395No Hit
AGCACACCTGTCAGTACGTACGAGTCGTAGAAGTTGTACTCGCAGTAGTC22923690.986331817947068No Hit
GACCGGTAGGTCAGTACGTACGAGTCAGACATATCGTACTCGCAGTAGTC17138490.7374134791810414No Hit
GCCATTAAGGTCAGTACGTACGAGTCTGACCAAGTGTACTCGCAGTAGTC16416030.7063283752909593No Hit
GCTGAGCAGGTCAGTACGTACGAGTCTGCATACATGTACTCGCAGTAGTC16365740.7041645601667555No Hit
CCGACTGTTGTCAGTACGTACGAGTCTATCTAGGTGTACTCGCAGTAGTC14482260.6231245420690171No Hit
GAGACATTGAGTACGTACGAGTCAATACGTCTGTACTCGCAGTAGTCCGA12770200.5494601690019212No Hit
GCGGTAGTCGTCAGTACGTACGAGTCAGGATGCGTGTACTCGCAGTAGTC12564760.5406207540264506No Hit
AAGGAAGTCGTCAGTACGTACGAGTCTCCGGAGCTGTACTCGCAGTAGTC12462890.5362376192739623No Hit
ACTTGATCTGTCAGTACGTACGAGTCGACGTCAATGTACTCGCAGTAGTC11809890.5081411532547727No Hit
ACTCCAACTGTCAGTACGTACGAGTCCGCGCACATGTACTCGCAGTAGTC11480450.4939664216079705No Hit
TCAGAGCGTAGTACGTACGAGTCGGTAGTGTTGTACTCGCAGTAGTCCGA10519730.45262976489440887No Hit
GCACAACTCTCAGTACGTACGAGTCGTCAGTTGTGTACTCGCAGTAGTCC10092880.434263797788297No Hit
CTCGCAAGAAGTACGTACGAGTCAATATCAGCGTACTCGCAGTAGTCCGA9989340.4298088083677351No Hit
TGAGTGACTGTCAGTACGTACGAGTCAGGAGCAATGTACTCGCAGTAGTC9040320.38897556459816196No Hit
GAAGTGTGACAGTACGTACGAGTCACAGCGTATGTACTCGCAGTAGTCCG8521270.36664253138643094No Hit
ACCATCTAACAGTACGTACGAGTCGCAACTCGCGTACTCGCAGTAGTCCG8170640.351556062963294No Hit
CCATGCGTCAGTACGTACGAGTCTAACGTGTCGTACTCGCAGTAGTCCGA7970720.34295415563319237No Hit
CAGTTGAGTTCAGTACGTACGAGTCATCCACAGTGTACTCGCAGTAGTCC7635330.32852341483966097No Hit
TCTACAGTTTCAGTACGTACGAGTCTGAGCAGATGTACTCGCAGTAGTCC7051480.3034022483996857No Hit
TCGCGTCATCAGTACGTACGAGTCTCCAGCAGCGTACTCGCAGTAGTCCG6936740.2984653594088101No Hit
GAGCACATTAGTACGTACGAGTCATGCCTGTCGTACTCGCAGTAGTCCGA6605010.2841920965105777No Hit
GCTGATGTCAGTACGTACGAGTCTGTCATAGTGTACTCGCAGTAGTCCGA6421070.2762777566031202No Hit
TGATGTGTGGTCAGTACGTACGAGTCTAAGTACACGTACTCGCAGTAGTC6360520.27367248705111114No Hit
GCGAAGCTCGTCAGTACGTACGAGTCAGGAGCAATGTACTCGCAGTAGTC6350100.27322414834372993No Hit
GGTTAGATGGTCAGTACGTACGAGTCTTCCTCGGTGTACTCGCAGTAGTC6335010.2725748747262267No Hit
AAGTCATGAGTCAGTACGTACGAGTCCAAGAGAGTGTACTCGCAGTAGTC6161520.265110164328571No Hit
ATTAGCGGTGTCAGTACGTACGAGTCGCCTTCCATGTACTCGCAGTAGTC5403580.23249847144253036No Hit
GCCTTGTGATCAGTACGTACGAGTCTCCATCATTGTACTCGCAGTAGTCC5320690.22893198435473458No Hit
CCAGTCTCACAGTACGTACGAGTCAGAGGACGTGTACTCGCAGTAGTCCG5250940.2259308687271106No Hit
CAACACTCCGTCAGTACGTACGAGTCGGACAGATTGTACTCGCAGTAGTC5165630.22226025691072918No Hit
GCTGATGTCAGTACGTACGAGTCTGTCATAGCGTACTCGCAGTAGTCCGA5111880.21994756924069636No Hit
CGAATGAGTTCAGTACGTACGAGTCTATCTAGGTGTACTCGCAGTAGTCC5104840.21964466093935625No Hit
GAATCACATGTCAGTACGTACGAGTCCTGACGGATGTACTCGCAGTAGTC4840440.2082683889401622No Hit
GATCGTTGATCAGTACGTACGAGTCGTAATCAGCGTACTCGCAGTAGTCC4802700.20664455949519403No Hit
AACGATGACGTCAGTACGTACGAGTCCCACAAGGTGTACTCGCAGTAGTC4542080.19543092860098088No Hit
ATGCGCCGTGTCAGTACGTACGAGTCCTGGTACATGTACTCGCAGTAGTC4534860.19512027548511787No Hit
CGGAGACTTGTCAGTACGTACGAGTCGCGGTAAGTGTACTCGCAGTAGTC4477120.19263591109316072No Hit
CGTTACACCCAGTACGTACGAGTCAATGCTGATGTACTCGCAGTAGTCCG4286840.18444878160750777No Hit
AGTTATCGCTCAGTACGTACGAGTCTTCCATACCGTACTCGCAGTAGTCC4053410.17440504797372613No Hit
TATTACTCGGTCAGTACGTACGAGTCTCATCATCTGTACTCGCAGTAGTC3995240.17190218208040872No Hit
TAGTCGCATCAGTACGTACGAGTCTAAGCCATTGTACTCGCAGTAGTCCG3992980.17180494163139898No Hit
CATGGTCGATCAGTACGTACGAGTCAGTGTCTCTGTACTCGCAGTAGTCC3948420.1698876697695076No Hit
GACCGGTAGGTCAGTACGTACGAGTCAGACATATTGTACTCGCAGTAGTC3887330.1672591657739298No Hit
ACATGACTAAGTACGTACGAGTCAACCGCTGAGTACTCGCAGTAGTCCGA3819180.1643268929420649No Hit
CAAGCGCATTCAGTACGTACGAGTCTCGTACCTTGTACTCGCAGTAGTCC3800090.16350551233516392No Hit
AGATCTTCAGTCAGTACGTACGAGTCCGCTGTAGTGTACTCGCAGTAGTC3739610.1609032546554693No Hit
ACACCACATCAGTACGTACGAGTCATCTAGGTTGTACTCGCAGTAGTCCG3620620.1557835019883585No Hit
ATCGATCCTTCAGTACGTACGAGTCCAACGTCTTGTACTCGCAGTAGTCC3567940.15351685293798958No Hit
GTGCGAGTAGTCAGTACGTACGAGTCTCGTGACGTGTACTCGCAGTAGTC3493230.15030232464351231No Hit
AGAATCAGAAGTACGTACGAGTCCATATGTCGGTACTCGCAGTAGTCCGA3464070.1490476646908024No Hit
CAGTAATGTCAGTACGTACGAGTCGCGTCGTCTGTACTCGCAGTAGTCCG3413380.14686663886765888No Hit
ATTCATGTGGTCAGTACGTACGAGTCCTGCTGCTTGTACTCGCAGTAGTC3340470.14372955871841064No Hit
ATCTCTGCTCAGTACGTACGAGTCCAGTACATCGTACTCGCAGTAGTCCG3182500.13693262343961835No Hit
AGAGCCGCATCAGTACGTACGAGTCAACGTTCTTGTACTCGCAGTAGTCC3150860.13557125715348178No Hit
CAGATCGACCAGTACGTACGAGTCATCTCTGCTGTACTCGCAGTAGTCCG3124350.13443061808124793No Hit
CGACCATCAGTCAGTACGTACGAGTCTCGCGTCATGTACTCGCAGTAGTC3013600.12966540581229655No Hit
TCCGATAACTCAGTACGTACGAGTCTGCAACTCTGTACTCGCAGTAGTCC2995910.1289042626516848No Hit
TTACGGACCGTCAGTACGTACGAGTCCTTCGTGCAGTACTCGCAGTAGTC2914650.1254079091620687No Hit
CAACACCTACAGTACGTACGAGTCCGTTCCATTGTACTCGCAGTAGTCCG2853640.12278284730627886No Hit
GATTGAGTAGTCAGTACGTACGAGTCGAATTCAGTGTACTCGCAGTAGTC2842460.12230180827091204No Hit
GCGTGATACGTCAGTACGTACGAGTCTAGCACTATGTACTCGCAGTAGTC2831970.12185045769121633No Hit
TGGAACTAACAGTACGTACGAGTCTCATATCGCGTACTCGCAGTAGTCCG2768240.119108363082636No Hit
CAGTTGAGTTCAGTACGTACGAGTCTACAGGACTGTACTCGCAGTAGTCC2751970.11840831790326048No Hit
TTCACTGGAGTCAGTACGTACGAGTCCAGCACGTGGTACTCGCAGTAGTC2732570.11757359900468119No Hit
GTGCGTTCCTCAGTACGTACGAGTCCCGATCTCTGTACTCGCAGTAGTCC2681790.11538870077427621No Hit
TTAAGTGGTGTCAGTACGTACGAGTCCACGGAAGTGTACTCGCAGTAGTC2644620.11378939657529723No Hit
TAGACCGCATCAGTACGTACGAGTCTCTCCGCGTGTACTCGCAGTAGTCC2597560.11176455784503221No Hit
TGTGTCTACGTCAGTACGTACGAGTCATCTGGTAGGTACTCGCAGTAGTC2508110.10791581529462407No Hit
ATCCGACCACAGTACGTACGAGTCATCTAGGTTGTACTCGCAGTAGTCCG2502230.10766281802020933No Hit
ATCTGCGTAAGTACGTACGAGTCGTCATTCTAGTACTCGCAGTAGTCCGA2335920.10050703966852262No Hit
AGTACTCTCTCAGTACGTACGAGTCGTAGAAGTTGTACTCGCAGTAGTCC2329120.10021445778654638No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)