FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007772656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174819594
Sequences flagged as poor quality0
Sequence length91-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAACCCAACATGAACTACGATAAGCTCAGCCGCGCCCTCCGTTACTA35318962.0203090049505548No Hit
TTTCTCTTTTGTTATTAGGAACAATCTCAGTTACAAAGTGAACTACTAAA29459791.685153782018279No Hit
GAAGACTTATCTCCTGCCTTTGATCATTCACCAAACAAAATAATGACTGA26240241.500989643071703No Hit
CCAAGTTTCCAGAAAACAGAAATCGAAACAGATACAGAGATGTAAGCCCA23406291.3388825282365089No Hit
TCGTACAAATTTACTCACCTGCAAACAGCCGATAGGTTCGGTGGCATTTA20888371.1948529064768334No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCCGTGGCCCCTGCACCA20503031.1728107548402156No Hit
GGAACACCAGGCAGATTCATTTATACAGGTAAGAACCACAGCACATGGTG19186921.0975268596036207No Hit
TGCTTGTAAGAGAGAGACCCAGATCCTTGGAGGATCTCCATCAGAAAAAA18104211.035593870558926No Hit
GCGTGTAGTAAATAGAATCCCTCAGTAGGCACCAGGAGGAAAGAGCCACA16835970.9630482267336692No Hit
GCATTCACCATAAATATCCAGGTTTGGTGTAGGCATAAGATACACGTTGA16488440.9431688761386781No Hit
GCTCTCTGTCAATCTCTCTGGTTGTGCTCAGAACTCCAGCAGTGCTCAGA16152800.9239696552550053No Hit
TGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTGAAAGA15891770.9090382626103113No Hit
TGGAGGAACATGCCGAGGAATTGAATTTCCAAGATGCAAAAATACTTGAA15360490.8786480764850649No Hit
CTTGTGCCTTCTGCTTCTCCCCTGCTGTTGCAGAGAGCCCTGCTGACGAA15297470.8750432174096No Hit
GCCATATAGATAAATCGGTACTGTGCTTCTGTCTGGACCATCCCTGACCT15225380.8709195377721791No Hit
TCAAGGGAATTGTTGAAGGACAGCATCAGCATGTAGCGTGCTAAAATAGC15177480.8681795703060607No Hit
CTAAAGAGGGAGCAACGCGAGGGTGGCTTACGGGTTGGTGGTGCTGAAGA15177070.8681561175573946No Hit
CGGTCTGAAGAGCCAAGAGCTATTTGATGAAATTAGAATGACCTACATCA14809350.8471218620951608No Hit
CAGCAACTCTGTGCCATTCCAGAGATCGCTAACAGTGAGGAGGTGCAGGA13940380.7974151913429108No Hit
TCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAACCAAGCCGAGT13931170.7968883625253128No Hit
TGTACACACCGATGTCATGGTCGTTCCTCAGGGTGCAGGTGAGGCGGATT13925950.7965897689935145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13817180.7903679263778637No Hit
GTCCCTGTCCGGCCAATTCCAGCACTGCGGGCAGAAGGACAAAAAGCAGG13366310.7645773390824829No Hit
CTGTTTGTTGGACATACTGGATACAGCTGGACAAGAAGAGTACAGTGCCA12977420.7423321209635116No Hit
TGTGACTTTGGATTGGCTCGAGATATCATGAGTGATTCCAACTATGTTGT12629440.7224270295468138No Hit
CAATTTACTTACCCCAGCAAGACATTTTTCCAGTGTATCCAATATAATCA12416810.7102642052812456No Hit
GCTATCCCAACAGATTGCCAAGCATGCCCACGGACATGGAACACACAGGA12260370.7013155516194598No Hit
TGGGACATCACCCTCACTTACCGGGTCACACTTGATCACTCTGGAGAGGA12216500.698806107512182No Hit
CATTCACACCAGAGTCATCATCCGTGGCTAAAATGGCTGCTACCAAAGAA12096450.6919390282990818No Hit
GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCG11902570.6808487382712947No Hit
TCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAATATTTCACCCTTCA11707980.669717834947037No Hit
AGGAATACCGGCCACGAACGTTCTGGTTTAACATGGCCGACGCCGCCTTC11306890.6467747545506827No Hit
TGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCCT11288520.6457239570067872No Hit
TTTACTTACTCTCGTCTCCACAGACACATACTCCATAATTTAAAACCAAA11169200.6388986351266781No Hit
AACGTGCCCAACTTTACTCGACTCAGGGGCTCGGACACCCCAGACATCCT10879140.6223066734727688No Hit
AGCAAGAGAAACTGGGCACAGTTTACTGTCAGGCAAGCTTTCCTGGAGCA10815990.6186943781599218No Hit
GCTTTGGGACTTGAGATTGTTTTTGTCTTTTTTCTGGCATTATTTCTGCT10715480.6129450226271548No Hit
GCTTTGGGACTTGAGATTGTTTTTGTCTTTTTTCTGGCATTATTTCTACT10643360.6088196269349533No Hit
AGAGACAGCGGGATTCCTTCCCACACCAGGATGAGAATCACAATCACTGC10286500.5884065833032424No Hit
TCTCATCAAAGGAATGCACTAGGATTCGCGCGGGGGTCTTTCTGGTTCCT10237730.5856168502484911No Hit
TCCACTCGGGCCACAAAGTTACTGGGCACATAGCCTTCTCTTCCTGTGAC10095360.5774730262787363No Hit
TTCTCCTTCCACCAGTTCCACGTTGCGCATCTCCTCCATGTAGCAGGCAT9992400.5715835262722324No Hit
CGTGAAACTGCTTCAGATGCTGCTCCATGGTCTCAATGATGCTCTTTTGC9968260.5702026741922304No Hit
CCGGACAAAAGTACAGCAGCCACCCAACCAACCAGTCCCAGCTTCCAGTC9967410.5701540526401176No Hit
TTGTAGTAGTGGCGCAGGGCTCTGGACATTTTCTCATAGGTCATGTTTGT9871530.5646695415618No Hit
ACACACATGGCAAAGACAAAGAAAGGAAAGCGTCTCTCGACCAAGGGAAA9579360.5479568840549991No Hit
AGCTTATTGAGTTCATCATTAAAGTCATCTATTTTCAACCTGGGGTACAA9489530.5428184440240721No Hit
AACGTGCCCAACTTTACTCGGCTCAGGGGCTCGGACACCCCAGACATCCT9486590.5426502706555879No Hit
GGTGTTTGTCTCCTCTTCATTGTCGTTTTAACCCTGCTAATTTGTCACAA9389070.5370719485826057No Hit
AAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATTGATGCCAAAGA9279970.5308312293643698No Hit
GTTCCATGTAATACTGGACCAACTCAGCCAAAGTGGCAAATTTCTCCCCT9200260.5262716718127145No Hit
GTGGTCTCAGCTAATTTGCTTAGTTCTCGAACTCTGCTTTCTATTTCAGC9172730.5246969055425217No Hit
ATCATGGCCCGTCACCTCCACCCCATCCAGACAAGCTTCCAGGAGGCTAC9112900.5212745202920447No Hit
ATCTCCTTTGTCCCCGCCACCTGAGGAATTGCCCACTTCCCCGCCACCTG9015230.51568761794516No Hit
GCTCCCCCTACTATTATAGCGCTGCCGCCCGAGGAGCCGCCCCACCTGCA8958490.5124419863370693No Hit
CCTTCATAGACCCTTCAGGCACATGCTTTTCTCTGGACGTTTACAGACAG8778840.5021656782934756No Hit
TGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGG8628380.4935590915512594No Hit
TCGTGGTTCACATCCCGCGGCTCACGGGGGAGTGGGCAGCGCCAGGGGCG8453900.4835785169481631No Hit
GTGGGTATTAAACAAGAACCAGATGACGGGAGCTATTACCCAGAGGCCAG8375050.4790681529668809No Hit
GGTTTCATTTTGCCAATTGAAGTTTATTTTAAAAACAAGGTATGTAATCT8162000.4668813039343862No Hit
AGTAACTTGAGGGGCACGACCGAGGGGGTTTACTACCAGGCCCCCGACTT8008760.45811569611584846No Hit
ACAGTTCTCTGAATGCCAATATGATTACTTCCAGATTAAAATGCAAAATT7928820.4535429821442098No Hit
CCATGATGACCACCAGTCCTACCAAGGACACCCTGCCTTAAATCCCAAGA7707960.44090938685053804No Hit
TCCACTCGGGCCACAAAGTTGCTGGGCACATAGCCTTCTCTTCCTGTGAC7686110.4396595269521104No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATCGAGCAC7521880.4302652710656678No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATTGAGCAC7439540.42555527271159316No Hit
TTCCTGGAGCAGCATTTGAATTACTGTCCTGTGACATAGCACGGACTTCC7309590.41812189542094463No Hit
CCAAGCCAAAACGACTGCAGGAGTGGTACAAAAAGATGCTGGACAAGGCG7228450.4134805392580879No Hit
GTCACTCTTGGAGCTTTGCTGTCCTCCCGAGGTGGTGGAGAGGTCCTCGG7111630.4067982219430163No Hit
TGATATGGAGGATGCTGGTAACAGATGTGGTCCCATTTGGGTGAGACAGA7090550.4055924074506202No Hit
TTGGAGAGCAGCAGCAGGTTCTCCACGGCGCTGTCCTGGGCCGAGTGGTT7034670.4023959694129023No Hit
TGCAGTACACCGGATTGGCCGCACCGGGCGCTCGGGAAACACAGGCATCG7000050.40041564219626313No Hit
CCCATCTGCCCAAGAGACTCTGTCAAAGTGATCAACAAGGAAGGGGAGGT6971250.39876822960703134No Hit
GTGGCAGCGCTCTTTATGTTCCTCTAAAGAGCTTCGCTGTTTATAGCTTC6895280.39442260688467223No Hit
CCCGAATGACCCCCAGATTTGACTCCAACAACGTGGTCCTCATTGAGGAC6772310.3873884983396083No Hit
CCCGAATGACCCCCAGATTCGACTCCAACAACGTGGTCCTCATTGAGGAC6700800.38329799576127604No Hit
GCTTTCAAAAGATTACATCAAATATGGACTCCGTGGGTCGAATACAAATG6535150.3738225132818922No Hit
CTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGATGT6417680.36710301477991075No Hit
ATGGTGTGGTTGACCTGGCCCAGCCCCTCGCTGCTCTCTGAGTGGGTGCA6214820.35549905235450896No Hit
CTTTAGCATTTACTAACATTCCAGATGTAAAAAGTGCCTCAGAACGACCC5955070.34064087804711407No Hit
AGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCG5903610.33769727208038247No Hit
ATTATGAGACAGGAAGCATCAAGCCTGGGGTAATTGGAGGATCCAAACCA5797060.3316024175184848No Hit
GGAAGCCACGAGAATATTGTGAACCTGCTGGGGGCGTGCACACTGTCAGG5651270.32326296330375875No Hit
AGGACCATTGCCTCAAGGGCATGAATTTGAACTGTACGAAGTGAGATTTC5595220.3200568009556183No Hit
AGCCTGTCTGAAGTGCAGTCTCTGAGCTCCTTCCAGTCCGAATCGTGCGA5582090.3193057409800414No Hit
GTGAGTGGATGGGTAAAACCTATCATCATAGGTCGTCATGCTTATGGGGA5398290.30879204535848537No Hit
TACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT5177530.2961641702474152No Hit
CATCACCAGGTGCGTTACCTGTTGGAATGGACAGAGGAGGACCTGGAGGA5073260.2901997358488317No Hit
CAGGAAATTACACACTGCCATGATGAAGATGATGATGAAGAGGAGGAGGA5072460.2901539743880197No Hit
GTCACAGTCAGCAGTAGCATATGCATGAGGTACAGTACTTTTAGATTCAG4902080.2804079272715849No Hit
CCGGCTGTCAGAAAGCAACTGATAGACGTAATCCCAAAGCAGTCTACAGT4851900.27753753964215244No Hit
GCTTCTTACCAGCATTCTGTGTGCTCCTGATGCAGCGAAGGCGTATCCCT4725240.27029235635909327No Hit
GCTCATGACTGCTAAGCTGAGCGCCTTGCTTGTGGCTGGAGCCTGGATGG4437410.25382795477719733No Hit
TGTCCAGCACGAGAGATCTCTCTGATCCTATCTTGCACAGGTCTTCTGCC4058860.2321742035392211No Hit
AGCTGGAGCGCATGAATCCCGGCCCAGGCGGGAAGCTGGGACACGCCCTG3767850.21552790015059753No Hit
CTCACTCACCACTGCAGCTGGCTTTGGAGGCAGCTCAGAGTTAGAGGTCT3616860.20689099644059353No Hit
ACATCCGAGTTAGAACTGAGGTTTGTTAATCTGGGGTCCGCATTGATTTC3491340.19971102323919138No Hit
GAAAGACGTCACACTGAGAGTATCTGGAGACCTTCATGTTGGAGGAGTGA3301840.18887127720934987No Hit
GATTCACCAGGTGCTCATGGACCATGGAGTCCCATGTCATTACACCTGCT3143580.17981851622421685No Hit
CAACATCTCCTTCGCAGCCGCGCAAAAAAAGTAGGCCATTTATTCATTAC3021040.1728090044643394No Hit
CGTGAAACTGCTTCAGATGCTGGAGAGATGAGCTGCTGCGACTACTGCGA2952620.16889525552839346No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCA2915990.1667999526414642No Hit
GGCTTCCGAATGGTAAACTGAAATGTGACGTCTGTGGCATGGTTTGCATT2748590.1572243669665541No Hit
TCCACCTGCCTTCAGAAGCCCAGGAGGTGGCCCTTGTGGCCTATAACTCA2730410.1561844377696015No Hit
GGATCCCAACGGAGTCAACCGTTTCGGGAGGCGCGCGATCCAGGTAGCTG2624110.15010388366420757No Hit
TGCTTGTAAGAGAGAGACCCAGGTCCTTGGAGGATCTCCATCAGAAAAAA2574760.1472809735503676No Hit
CTAGGTGGTGCTGAGAGAGGCGTGGGGTGGCTGAGTGGTCCAGAAGCAGC2411510.1379427754534197No Hit
AGATGTACCTATGGTCCTAGTAGGAAATAAATGTGATTTGCCTTCTAGAA2347100.1342584058397939No Hit
TCTCGAGAACTCTGTGTGCCACTGGGAGTGCCTCTAGACCGTTCATATCC2251970.12881679613098745No Hit
ACAATGAGCTTTCACCCGAAAGGGAGCACTCCAATATGGCAATTGACCTC2249080.12865148285380412No Hit
GCGTGTAGTAAATAGAATCCCTCAGCAGGCACCAGGAGGAAAGAGCCACA2208830.1263491093567006No Hit
GTCTCTCTCTCTCTCTTTAGGCCAAGGCAGGTGGATCACCTGAGGTCAGG2038350.11659734205766432No Hit
ATTTGCCAACAAGGACAGTTGATACAAAACAAGCCCACGAACTGGCCAAG2025640.11587030684901373No Hit
GCTTCACTGCCGTTCTTGGCCATTCCCTAGGAATAGAGGAAAAGAACAAG2007170.11481378912251679No Hit
CGTTATGTGCGACGAGAACTCGTACCGGTCCTGGCTGTGGTAGCCGCACA1907460.10911019505056166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)