FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007772657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195056963
Sequences flagged as poor quality0
Sequence length138-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGAGATCAGTACGTACGAGTCTCACTCGCTGTACTCGCAGTAGTCC37859381.9409396833477817No Hit
ATGGAGGAAGTCAGTACGTACGAGTCGATTCCTCTGTACTCGCAGTAGTC22159321.1360435259109412No Hit
CAGCGTAAGGTCAGTACGTACGAGTCTATGTACCTGTACTCGCAGTAGTC22096661.1328311309758268No Hit
CATCTCGATTCAGTACGTACGAGTCATCGGTGGTGTACTCGCAGTAGTCC20269831.039174899898344No Hit
ACACCACATCAGTACGTACGAGTCTAAGCCATTGTACTCGCAGTAGTCCG15911930.8157581126698871No Hit
GGTGCGTAGTCAGTACGTACGAGTCCGCGAGTGTGTACTCGCAGTAGTCC14514510.7441164763751602No Hit
CGCTTATGACAGTACGTACGAGTCAGGAGCAATGTACTCGCAGTAGTCCG12852330.658901369237457No Hit
ACATTCCACAGTACGTACGAGTCACCGACGGTGTACTCGCAGTAGTCCGA12720090.6521218112065038No Hit
TACGAGTGACAGTACGTACGAGTCAATCGCCATGTACTCGCAGTAGTCCG12470420.6393219605290379No Hit
GGTCTTGCAGTCAGTACGTACGAGTCCGCGCACATGTACTCGCAGTAGTC12317340.6314739966498915No Hit
AACTAACTGTCAGTACGTACGAGTCCAGCTAGTTGTACTCGCAGTAGTCC11438560.5864215162624059No Hit
CTCCTGACGCAGTACGTACGAGTCTCAACACTCGTACTCGCAGTAGTCCG11221420.5752893835427961No Hit
GTCAGACCGGTCAGTACGTACGAGTCCGACCACTTGTACTCGCAGTAGTC11211750.5747936309251365No Hit
GTTCAGAAGAGTACGTACGAGTCAGGATGCGTGTACTCGCAGTAGTCCGA10792150.5532819661505751No Hit
CATTATGTCGTCAGTACGTACGAGTCCGCACTCTTGTACTCGCAGTAGTC9383270.4810528091734926No Hit
CCGTTCCAACAGTACGTACGAGTCGGTAAGGATGTACTCGCAGTAGTCCG9093210.46618228132671174No Hit
CTTACATGCGTCAGTACGTACGAGTCCCGATCTCTGTACTCGCAGTAGTC8820770.45221507934582167No Hit
AACGGTAGCAGTACGTACGAGTCGGACTCCTTGTACTCGCAGTAGTCCGA7816530.4007306316975724No Hit
ATTGCGCTATCAGTACGTACGAGTCCGCCACCATGTACTCGCAGTAGTCC7347060.37666227788033385No Hit
AATCTGACCGTCAGTACGTACGAGTCGTAGACTCTGTACTCGCAGTAGTC6711360.344071798144422No Hit
GTAGTTGGTAGTACGTACGAGTCCCGTCAGGTGTACTCGCAGTAGTCCGA6454450.33090077384215194No Hit
CGGAGACTTGTCAGTACGTACGAGTCCAATTGTCTGTACTCGCAGTAGTC6442660.33029633502496397No Hit
GTGCATCAACAGTACGTACGAGTCCCTAAGTCTGTACTCGCAGTAGTCCG6138960.3147265242717841No Hit
CATCGAAGCAGTACGTACGAGTCTTCCTTGACGTACTCGCAGTAGTCCGA6118770.31369144202250293No Hit
GAGTCACCAGTCAGTACGTACGAGTCCATGACGTTGTACTCGCAGTAGTC6114700.3134827850262387No Hit
ATCCAATGAAGTACGTACGAGTCTAGATCTGTGTACTCGCAGTAGTCCGA5971340.3061331371185145No Hit
CTTCCGTGGGTCAGTACGTACGAGTCTGAACCAATGTACTCGCAGTAGTC5923860.3036989763856828No Hit
ACATTGACACAGTACGTACGAGTCGTAGAAGTTGTACTCGCAGTAGTCCG5770190.2958207649321394No Hit
GACGGTGCTAGTACGTACGAGTCGAGTTAACTGTACTCGCAGTAGTCCGA5658490.2900942326268045No Hit
CAGCTCTTCCAGTACGTACGAGTCGGTAGACATGTACTCGCAGTAGTCCG5280460.27071374017035216No Hit
GACGTCAATGTCAGTACGTACGAGTCATGGAGAGTGTACTCGCAGTAGTC5138100.26341535933787713No Hit
ATCATGTTCGTCAGTACGTACGAGTCTGACCTCCTGTACTCGCAGTAGTC4615950.23664625599651112No Hit
TAAGCACTATCAGTACGTACGAGTCAGTCCGTGTGTACTCGCAGTAGTCC4605980.23613512325627667No Hit
TTCCAATTCTCAGTACGTACGAGTCACGGTGCCTGTACTCGCAGTAGTCC4526390.23205477673719344No Hit
CAAGGTGTGCAGTACGTACGAGTCGCGCTCTATGTACTCGCAGTAGTCCG4427840.22700240647138548No Hit
TGTTACTGACAGTACGTACGAGTCTCGCGTCATGTACTCGCAGTAGTCCG4270830.21895296298650976No Hit
GGAAGAAGCAGTACGTACGAGTCGACCTTGTGGTACTCGCAGTAGTCCGA4074620.20889385015186562No Hit
TTCAACTACTCAGTACGTACGAGTCGCCTTCCATGTACTCGCAGTAGTCC4026030.20640278296550738No Hit
TAATGTAGCAGTACGTACGAGTCGACGGTGCTGTACTCGCAGTAGTCCGA3919530.20094283945146832No Hit
GCACCATCGGTCAGTACGTACGAGTCAGTTCGATAGTACTCGCAGTAGTC3823500.19601966221528835No Hit
TGTAGCAAGCAGTACGTACGAGTCGCCTTCCATGTACTCGCAGTAGTCCG3542870.1816325828881074No Hit
CGCTTATGACAGTACGTACGAGTCTTAAGTGGTGTACTCGCAGTAGTCCG3439160.17631567451401364No Hit
CGCGCACATTCAGTACGTACGAGTCGCAGCTCTTGTACTCGCAGTAGTCC3410970.1748704556627389No Hit
TATCGGAACCAGTACGTACGAGTCTAGACCATGGTACTCGCAGTAGTCCG3219430.1650507600695085No Hit
AATTGGTGATCAGTACGTACGAGTCGTCGACATTGTACTCGCAGTAGTCC2996700.15363204439925582No Hit
TGGTAACCATCAGTACGTACGAGTCCCTCCTTGTGTACTCGCAGTAGTCC2961290.1518166772646819No Hit
AGAAGTAACTCAGTACGTACGAGTCTTAACGTTCGTACTCGCAGTAGTCC2916870.14953939378211276No Hit
GATGTTACAAGTACGTACGAGTCATCGGTGGTGTACTCGCAGTAGTCCGA2896350.14848739339799932No Hit
CCGATACCGAGTACGTACGAGTCAGTGACGATGTACTCGCAGTAGTCCGA2887110.1480136856226968No Hit
AGTCGCGACGTCAGTACGTACGAGTCAATCGCCATGTACTCGCAGTAGTC2874690.14737694854810182No Hit
CTGTCACTTTCAGTACGTACGAGTCGTCCTTCATGTACTCGCAGTAGTCC2856850.14646234392565624No Hit
GTAAGGTCCTCAGTACGTACGAGTCGTGTACCGTGTACTCGCAGTAGTCC2733790.14015341764549055No Hit
TACCTCCTCAGTACGTACGAGTCGCGCTCTATGTACTCGCAGTAGTCCGA2722020.13955000416980756No Hit
CTTCTCATCAGTACGTACGAGTCAACGGAGATGTACTCGCAGTAGTCCGA2705000.1386774385490663No Hit
GTCGCAGGTTCAGTACGTACGAGTCACTAACCGAGTACTCGCAGTAGTCC2643070.13550246857888382No Hit
TAGATAGCCGTCAGTACGTACGAGTCCGTTGATCCGTACTCGCAGTAGTC2611580.1338880683792867No Hit
ATGAGCAGGTCAGTACGTACGAGTCCGCGAGTGTGTACTCGCAGTAGTCC2604200.13350971736394768No Hit
ATGAACTAGAGTACGTACGAGTCCTGGACACTGTACTCGCAGTAGTCCGA2375490.12178442458370481No Hit
AAGCACAACAGTACGTACGAGTCTACATGCACGTACTCGCAGTAGTCCGA2308980.118374651408881No Hit
GCGACAACCTCAGTACGTACGAGTCATGGAGAGTGTACTCGCAGTAGTCC2301040.1179675908314024No Hit
CCACGCCATGTCAGTACGTACGAGTCCTTCTGAGTGTACTCGCAGTAGTC2286730.11723395898458648No Hit
CCACACAGAAGTACGTACGAGTCTTGATGAGTGTACTCGCAGTAGTCCGA2278190.11679613816195836No Hit
TAGGATTAGTCAGTACGTACGAGTCGGATACAATGTACTCGCAGTAGTCC2254850.1155995646256422No Hit
TCCTGTCCGTCAGTACGTACGAGTCCTCTGCGGTGTACTCGCAGTAGTCC2243670.11502639872435622No Hit
CAGTGCCATTCAGTACGTACGAGTCGATCGCACTGTACTCGCAGTAGTCC2234190.11454038685099388No Hit
CAATCTCCGAGTACGTACGAGTCACCATGTCTGTACTCGCAGTAGTCCGA2213160.11346224025850335No Hit
CTCGAACTGTCAGTACGTACGAGTCTCAGAGCGTGTACTCGCAGTAGTCC2153020.1103790383530169No Hit
GCCACATGGCAGTACGTACGAGTCTGCGACGTTGTACTCGCAGTAGTCCG2093390.10732198265590756No Hit
TCAAGGTCGGTCAGTACGTACGAGTCCGTACATCTGTACTCGCAGTAGTC2012040.10315140608438572No Hit
GTTGACCATTCAGTACGTACGAGTCCACCTTCCTGTACTCGCAGTAGTCC1983580.10169234512279368No Hit
GACCAGAGACAGTACGTACGAGTCCACTGTATCGTACTCGCAGTAGTCCG1975310.10126836641048287No Hit
GCTGAGCAGGTCAGTACGTACGAGTCTATCTAGGTGTACTCGCAGTAGTC1974160.10120940927394631No Hit
ACGCACACTTCAGTACGTACGAGTCCGCCATTACGTACTCGCAGTAGTCC1958560.10040964290005888No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)