FastQCFastQC Report
Thu 12 Jan 2023
EGAF00007772659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24788715
Sequences flagged as poor quality0
Sequence length139-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCTGCGTCAGTACGTACGAGTCGTCCGCTCAGTACTCGCAGTAGTC480160.19370104501181284No Hit
ATGTGACGAGTCAGTACGTACGAGTCCATACAACCGTACTCGCAGTAGTC363340.1465747619430858No Hit
ATTCCGTACGTCAGTACGTACGAGTCCACCAAGATGTACTCGCAGTAGTC342200.1380466877770792No Hit
CGAGCTTACGTCAGTACGTACGAGTCTTAACGGAGGTACTCGCAGTAGTC334390.13489606056626977No Hit
TTGCTCTGCGTCAGTACGTACGAGTCCTTCACTGTGTACTCGCAGTAGTC325240.13120486479432275No Hit
TATGTATGCGTCAGTACGTACGAGTCTATGACCGAGTACTCGCAGTAGTC309850.12499639452872002No Hit
TCTCCGCGTGTCAGTACGTACGAGTCGCCATACAAGTACTCGCAGTAGTC308660.12451633737367993No Hit
TATTGACTGGTCAGTACGTACGAGTCAGTGAGAGAGTACTCGCAGTAGTC301160.1214907670688053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)