FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007772660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007772660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24788715
Sequences flagged as poor quality0
Sequence length138-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTTACTTACCCCAGCAAGACATTTTTCCAGTGTATCCAATATAATCA4965402.0030889055765897No Hit
TTTCTCTTTTGTTATTAGGAACAATCTCAGTTACAAAGTGAACTACTAAA4956321.999425948460822No Hit
TCAAGGGAATTGTTGAAGGACAGCATCAGCATGTAGCGTGCTAAAATAGC4320571.7429584389509498No Hit
GAAGACTTATCTCCTGCCTTTGATCATTCACCAAACAAAATAATGACTGA4116011.6604370174089298No Hit
TGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTGAAAGA3777041.5236933419098166No Hit
TGGAGGAACATGCCGAGGAATTGAATTTCCAAGATGCAAAAATACTTGAA3653671.473924727441499No Hit
CCGGCTGTCAGAAAGCAACTGATAGACGTAATCCCAAAGCAGTCTACAGT3614191.457998125356639No Hit
TGCTTGTAAGAGAGAGACCCAGGTCCTTGGAGGATCTCCATCAGAAAAAA3380801.3638464115626807No Hit
TGCTTGTAAGAGAGAGACCCAGATCCTTGGAGGATCTCCATCAGAAAAAA3008861.2138023290033388No Hit
GTCCCTGTCCGGCCAATTCCAGCACTGCGGGCAGAAGGACAAAAAGCAGG3004031.2118538617269996No Hit
GGCTTCCGAATGGTAAACTGAAATGTGACGTCTGTGGCATGGTTTGCATT2827931.1408134709685436No Hit
TTTACTTACTCTCGTCTCCACAGACACATACTCCATAATTTAAAACCAAA2670491.0773006991286156No Hit
TCGTACAAATTTACTCACCTGCAAACAGCCGATAGGTTCGGTGGCATTTA2624271.0586551178631083No Hit
CTTGTGCCTTCTGCTTCTCCCCTGCTGTTGCAGAGAGCCCTGCTGACGAA2565561.0349709535165497No Hit
TGTGACTTTGGATTGGCTCGAGATATCATGAGTGATTCCAACTATGTTGT2520871.0169425885932368No Hit
GTGGTCTCAGCTAATTTGCTTAGTTCTCGAACTCTGCTTTCTATTTCAGC2396240.9666656783137004No Hit
TTGTAGTAGTGGCGCAGGGCTCTGGACATTTTCTCATAGGTCATGTTTGT2278830.9193013837143234No Hit
CATTCACACCAGAGTCATCATCCGTGGCTAAAATGGCTGCTACCAAAGAA2133130.860524637924959No Hit
AGCAAACCCAACATGAACTACGATAAGCTCAGCCGCGCCCTCCGTTACTA2132640.8603269673317072No Hit
CCAAGTTTCCAGAAAACAGAAATCGAAACAGATACAGAGATGTAAGCCCA2074210.8367557576098641No Hit
CTGTTTGTTGGACATACTGGATACAGCTGGACAAGAAGAGTACAGTGCCA1913380.7718754279921327No Hit
ATCTCCTTTGTCCCCGCCACCTGAGGAATTGCCCACTTCCCCGCCACCTG1909270.7702174154650614No Hit
GGAACACCAGGCAGATTCATTTATACAGGTAAGAACCACAGCACATGGTG1880900.7587726915251557No Hit
CGTGAAACTGCTTCAGATGCTGCTCCATGGTCTCAATGATGCTCTTTTGC1863020.7515597319183346No Hit
GGTTTCATTTTGCCAATTGAAGTTTATTTTAAAAACAAGGTATGTAATCT1799740.7260319867326724No Hit
ATGGTGTGGTTGACCTGGCCCAGCCCCTCGCTGCTCTCTGAGTGGGTGCA1785690.7203640850282074No Hit
AGCTTATTGAGTTCATCATTAAAGTCATCTATTTTCAACCTGGGGTACAA1773070.7152730587285384No Hit
AGCAAGAGAAACTGGGCACAGTTTACTGTCAGGCAAGCTTTCCTGGAGCA1770830.7143694217308159No Hit
GCCATATAGATAAATCGGTACTGTGCTTCTGTCTGGACCATCCCTGACCT1724210.6955624767157151No Hit
GCTCCCCCTACTATTATAGCGCTGCCGCCCGAGGAGCCGCCCCACCTGCA1652740.66673080875713No Hit
TTCCTGGAGCAGCATTTGAATTACTGTCCTGTGACATAGCACGGACTTCC1538120.6204920263111662No Hit
TGCTTGTAAGAGAGAGACCCAGATCCTTGGAGGATCTCCATCAGAAAATA1526030.6156148069797083No Hit
GCTCTCTGTCAATCTCTCTGGTTGTGCTCAGAACTCCAGCAGTGCTCAGA1513570.6105883261798766No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCA1505820.6074619035315062No Hit
GCTTTGGGACTTGAGATTGTTTTTGTCTTTTTTCTGGCATTATTTCTACT1499430.604884117631753No Hit
GCTTTGGGACTTGAGATTGTTTTTGTCTTTTTTCTGGCATTATTTCTGCT1473540.5944398489393259No Hit
CTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGATGT1466670.5916684265400607No Hit
GGTGTTTGTCTCCTCTTCATTGTCGTTTTAACCCTGCTAATTTGTCACAA1462370.5899337662319325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1454020.5865652979591722No Hit
GCATTCACCATAAATATCCAGGTTTGGTGTAGGCATAAGATACACGTTGA1448880.5844917737768981No Hit
CCCATCTGCCCAAGAGACTCTGTCAAAGTGATCAACAAGGAAGGGGAGGT1424650.5747171646452831No Hit
TGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCCT1417050.5716512534030103No Hit
TTCTCCTTCCACCAGTTCCACGTTGCGCATCTCCTCCATGTAGCAGGCAT1415590.5710622757169946No Hit
CCGGACAAAAGTACAGCAGCCACCCAACCAACCAGTCCCAGCTTCCAGTC1360900.5489998170538489No Hit
AAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATTGATGCCAAAGA1327460.5355098075878479No Hit
TTGGAGAGCAGCAGCAGGTTCTCCACGGCGCTGTCCTGGGCCGAGTGGTT1285670.5186513298490866No Hit
AGGAATACCGGCCACGAACGTTCTGGTTTAACATGGCCGACGCCGCCTTC1284280.5180905908192498No Hit
CCAAGCCAAAACGACTGCAGGAGTGGTACAAAAAGATGCTGGACAAGGCG1266480.5109099039623474No Hit
CCATGATGACCACCAGTCCTACCAAGGACACCCTGCCTTAAATCCCAAGA1256020.5066902419104823No Hit
ACAGTTCTCTGAATGCCAATATGATTACTTCCAGATTAAAATGCAAAATT1244230.5019340453912193No Hit
AGTAACTTGAGGGGCACGACCGAGGGGGTTTACTACCAGGCCCCCGACTT1243840.5017767157353659No Hit
AGGACCATTGCCTCAAGGGCATGAATTTGAACTGTACGAAGTGAGATTTC1219290.4918730156040763No Hit
ACACACATGGCAAAGACAAAGAAAGGAAAGCGTCTCTCGACCAAGGGAAA1212210.4890168772362746No Hit
ACAGTTCTCTGAATGCCAATATGATTATTTCCAGATTAAAATGCAAAATT1198890.4836434643748173No Hit
CTAAAGAGGGAGCAACGCGAGGGTGGCTTACGGGTTGGTGGTGCTGAAGA1187720.4791373816674241No Hit
GTGGCAGCGCTCTTTATGTTCCTCTAAAGAGCTTCGCTGTTTATAGCTTC1175870.4743569805857222No Hit
ATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCCGTGGCCCCTGCACCA1158690.4674264075406893No Hit
TCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAATATTTCACCCTTCA1155810.46626458854361746No Hit
AGCCTGTCTGAAGTGCAGTCTCTGAGCTCCTTCCAGTCCGAATCGTGCGA1147200.4627912338336215No Hit
TGCTTGTAAGAGAGAGACCCAGGTCCTTGGAGGATCTCCATCAGAAAATA1131230.45634878613110846No Hit
GCGTGTAGTAAATAGAATCCCTCAGTAGGCACCAGGAGGAAAGAGCCACA1128190.45512242163419925No Hit
GTTCCATGTAATACTGGACCAACTCAGCCAAAGTGGCAAATTTCTCCCCT1079090.4353150213716201No Hit
TGGGACATCACCCTCACTTACCGGGTCACACTTGATCACTCTGGAGAGGA1063510.42902990332496055No Hit
ATTATGAGACAGGAAGCATCAAGCCTGGGGTAATTGGAGGATCCAAACCA1026100.41393835864424594No Hit
GGCCCAGAAGAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCG1000800.4037321014824689No Hit
GTGAGTGGATGGGTAAAACCTATCATCATAGGTCGTCATGCTTATGGGGA971660.39197675232459606No Hit
CAGCAACTCTGTGCCATTCCAGAGATCGCTAACAGTGAGGAGGTGCAGGA964540.38910447758183514No Hit
AGAGACAGCGGGATTCCTTCCCACACCAGGATGAGAATCACAATCACTGC950060.3832631098465572No Hit
GCGTGTAGTAAATAGAATCCCTCAGCAGGCACCAGGAGGAAAGAGCCACA947950.3824119160674525No Hit
GCTATCCCAACAGATTGCCAAGTATGCCCACGGACATGGAACACACAGGA937890.37835361776518067No Hit
TGTCCAGCACGAGAGATCTCTCTGATCCTATCTTGCACAGGTCTTCTGCC934490.3769820258936375No Hit
GTCACTCTTGGAGCTTTGCTGTCCTCCCGAGGTGGTGGAGAGGTCCTCGG933630.3766350938320119No Hit
TCTCATCAAAGGAATGCACTAGGATTCGCGCGGGGGTCTTTCTGGTTCCT889020.3586390016586177No Hit
GCTTCTTACCAGCATTCTGTGTGCTCCTGATGCAGCGAAGGCGTATCCCT861390.3474928006554595No Hit
TCGTGGTTCACATCCCGCGGCTCACGGGGGAGTGGGCAGCGCCAGGGGCG853580.3443421734446501No Hit
TGCAGTACACCGGATTGGCCGCACCGGGCGCTCGGGAAACACAGGCATCG853140.3441646733200975No Hit
GCTATCCCAACAGATTGCCAAGCATGCCCACGGACATGGAACACACAGGA790220.31878215550906935No Hit
AGATGTACCTATGGTCCTAGTAGGAAATAAATGTGATTTGCCTTCTAGAA787300.31760420013703816No Hit
AGCTTATTGAGTTCATCATTAAAGTCATCTATTTTCAACCTGGGGTACTA779950.31463914123826103No Hit
CATCACCAGGTGCGTTACCTGTTGGAATGGACAGAGGAGGACCTGGAGGA768780.31013305853086776No Hit
TCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAACCAAGCCGAGT766630.3092657283768037No Hit
CAACATCTCCTTCGCAGCCGCGCAAAAAAAGTAGGCCATTTATTCATTAC765530.3088219780654221No Hit
GTCACAGTCAGCAGTAGCATATGCATGAGGTACAGTACTTTTAGATTCAG756220.30506623679363776No Hit
GCTTTCAAAAGATTACATCAAATATGGACTCTGTGGGTCGAATACAAATG742740.29962827843234313No Hit
CCCATTTCTTCTCCTTACTGCAGGTGAACGCCCCTTCCACTGTAACCAGT716810.2891678733649566No Hit
GCGTTCACCATAAATATCCAGGTTTGGTGTAGGCATAAGATACACGTTGA706170.28487559762577447No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATTGAGCAC700840.2827254256624436No Hit
TCTCCCCGGCCAAAGAAGACACCACTGGATGGAGAATATTTCACCCTTCA697850.2815192316342336No Hit
CGGTCTGAAGAGCCAAGAGCTATTTGATGAAATTAGAATGACCTACATCA690980.27874780923496845No Hit
AGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCG684840.27627087567871106No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATCGAGCAC670000.27028428056879916No Hit
AGCCTGTCTGAAGTGCAGTCCCTGAGCTCCTTCCAGTCCGAATCGTGCGA669510.27008660997554734No Hit
AACGTGCCCAACTTTACTCGACTCAGGGGCTCGGACACCCCAGACATCCT667770.26938467766481644No Hit
GCTTTCAAAAGATTACATCAAATATGGACTCCGTGGGTCGAATACAAATG639330.25791171506873184No Hit
TACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT637940.2573509760388951No Hit
AACGTGCCCAACTTTACTCGGCTCAGGGGCTCGGACACCCCAGACATCCT631950.25493455388873526No Hit
AGCTGGAGCGCATGAATCCCGGCCCAGGCGGGAAGCTGGGACACGCCCTG630820.25447870129613415No Hit
TGGCTCTGACTGTACCACCCTCCACTACAACTACATGTGTAACAGTTCCT618470.2494965955274406No Hit
TCCACTCGGGCCACAAAGTTACTGGGCACATAGCCTTCTCTTCCTGTGAC614460.24787892393776764No Hit
TGTACACACCGATGTCATGGTCGTTCCTCAGGGTGCAGGTGAGGCGGATT600370.2421948858583432No Hit
GTGGGTATTAAACAAGAACCAGATGACGGGAGCTATTACCCAGAGGCCAG597220.24092414633029588No Hit
TGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGG591260.2385198264613555No Hit
GGAAGCCACGAGAATATTGTGAACCTGCTGGGGGCGTGCACACTGTCAGG580560.2342033461597344No Hit
CCCGAATGACCCCCAGATTTGACTCCAACAACGTGGTCCTCATTGAGGAC575700.23224277660217563No Hit
GATTCACCAGGTGCTCATGGACCATGGAGTCCCATGTCATTACACCTGCT551740.2225770880015362No Hit
GCCATATAGATAAATCGGTCCTGTGCTTCTGTCTGGACCATCCCTGACCT498990.20129724352391803No Hit
TTTCTCTTTTGTTATTAGGAACAATCTCAGTTACAAAGTGAACTACTATA493050.19890099184245733No Hit
TCGTGGTTCACATCCCGCGGCTCACGGGGGAGTGGGCCGCGCCAGGGGCG492700.1987597985615632No Hit
TGATATGGAGGATGCTGGTAACAGATGTGGTCCCATTTGGGTGAGACAGA487980.19685570631636212No Hit
TCCACCTGCCTTCAGAAGCCCAGGAGGTGGCCCTTGTGGCCTATAACTCA483870.1951976937892908No Hit
TGATATGGAGGATGCTGGTAACAGACGTGGTCCCATTTGGGTGAGACAGA480710.19392292016750365No Hit
CTCACTCACCACTGCAGCTGGCTTTGGAGGCAGCTCAGAGTTAGAGGTCT480020.19364456769945518No Hit
TCCACTCGGGCCACAAAGTTGCTGGGCACATAGCCTTCTCTTCCTGTGAC465610.18783143862035606No Hit
CCCGAATGACCCCCAGATTCGACTCCAACAACGTGGTCCTCATTGAGGAC457420.184527515847433No Hit
CTTTAGCATTTACTAACATTCCAGATGTAAAAAGTGCCTCAGAACGACCC435900.17584614611931276No Hit
GGGATAGAGGAGCGGATGACCGTCGTCTGGGACAAGGCGGTGGTAAGGCT434160.17514421380858186No Hit
TTTACTTACTCTCGTCTCCCCAGACACATACTCCATAATTTAAAACCAAA429250.17316347378232394No Hit
TCGTGCAAATTTACTCACCTGCAAACAGCCGATAGGTTCGGTGGCATTTA422560.17046466507037578No Hit
CGTTATGTGCGACGAGAACTCGTACCGGTCCTGGCTGTGGTAGCCGCACA416530.1680321065452566No Hit
CAACATCTCCTTCGCAGCCGCGCAAAAAAAGTAGGCCATTTATTCATTTC415300.16753591301525714No Hit
GATTCACCAGGTGCTCATGGACCGGGAGGACACGTGTCACCGCACCTGCT408950.1649742634904633No Hit
CCCGAATGACCCCCAGATTCGACTCCAACAACGTGGTCCTCATTGAGGTC404640.16323556908859535No Hit
GTCTCTCTCTCTCTCTTTAGGCCAAGGCAGGTGGATCACCTGAGGTCAGG401970.16215846606005999No Hit
GGTGTTTGTCTCCTCTTCATTGTCGTTTTAACCCTGCTAATTTGTCACTA392500.15833817928843832No Hit
CCCGAATGACCCCCAGATTTGACTCCAACAACGTGGTCCTCATTGAGGTC389650.15718846257258595No Hit
AGGTGATCAGTTGGTCCTTCTCGGTCCTTTGATTGTTTAATAAGATCAAA385920.1556837456076283No Hit
TCTCGAGAACTCTGTGTGCCACTGGGAGTGCCTCTAGACCGTTCATATCC383090.15454209707925562No Hit
GTGGGTATTAAACAAGAACCAGATGACGGGAGCTATTACCCAGAGGCCTG376290.1517989133361693No Hit
GAAAGACGTCACACTGAGAGTATCTGGAGACCTTCACGTTGGAGGAGTGA375980.15167385643023448No Hit
GAAAGACGTCACACTGAGAGTATCTGGAGACCTTCATGTTGGAGGAGTGA375370.1514277767121047No Hit
CAGGAAATTACACACTGCCATGATGAAGATGATGATGAAGAGGAGGAGGA369760.14916465012405847No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATCGAGCTC346830.1399144731786218No Hit
CCTGCGAGTGTGGCAAAGCCTTCCGGCACCGGTCAGCCCTCATTGAGCTC341460.13774816484033156No Hit
TCAAGGGAATTGTTGAAGGCCAGCATCAGCATGTAGCGTGCTAAAATAGC336290.135662538376838No Hit
ATCATGGCCCGTCACCTCCACCCCATCCAGACAAGCTTCCAGGAGGCTAC329920.1330928206645645No Hit
ACATCCGAGTTAGAACTGAGGTTTGTTAATCTGGGGTCCGCATTGATTTC305290.12315684778335625No Hit
TTCTCCTTCCACCAGTTCCCCGTTGCGCATCTCCTCCATGTAGCAGGCAT304450.1228179839092103No Hit
ATCATGGCCCGTCACCTCCACCCCATCCAGACCAGCTTCCAGGAGGCTAC290590.117226729985802No Hit
ACGCGCATGGCAAAGACAAAGAAAGGAAAGCGTCTCTCGACCAAGGGATA285400.11513303533482878No Hit
CAAGACTTATCTCCTGCCTTTGATCATTCACCAAACAAAATAATGACTGA283630.11441900074287838No Hit
CTAGGTGGTGCTGAGAGAGGTGTGGGGTGGCTGAGTGGTCCAGAAGCAGC273560.11035666834686671No Hit
ATTTGCCAACAAGGACAGTTGATACAAAACAAGCCCACGAACTGGCCAAG265390.10706081376142329No Hit
CTAGGTGGTGCTGAGAGAGGCGTGGGGTGGCTGAGTGGTCCAGAAGCAGC254770.10277660620972083No Hit
TCGTGGTTCACATACCGCGGCTCACGGGGGAGTGGGCCGCGCCAGGGGCG251550.10147762802549466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)