FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9434983
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99492510.545064045160442Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA546220.5789305608711748No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT270450.2866459854776633Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCACCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115720.12264992952292547Clontech SMART CDS Primer II A (96% over 26bp)
ATGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107750.11420264350237833Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106580.11296257767502071Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)