FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10945572
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC221859320.269319867431324TruSeq Adapter, Index 7 (100% over 50bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7885257.2040547538310475Clontech SMART CDS Primer II A (100% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC3507163.204181563101499TruSeq Adapter, Index 7 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA299150.27330686783660096No Hit
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGC190120.17369581050675104TruSeq Adapter, Index 7 (98% over 50bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123480.11281274290644654Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)