FastQCFastQC Report
Sun 2 Apr 2023
EGAF00007808269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20634693
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210792310.215431845775463Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3508101.7000979854655456No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT401660.1946527627040538Clontech SMART CDS Primer II A (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC332460.16111700813770286TruSeq Adapter, Index 10 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC328280.15909129348326143TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)