FastQCFastQC Report
Sat 1 Apr 2023
EGAF00007808282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17434409
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15580518.936643622390642Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1389400.7969297955554445TruSeq Adapter, Index 4 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA863080.4950440247214575No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC325310.18659078148275632TruSeq Adapter, Index 4 (100% over 50bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265300.15217034314154268Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)