FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11124494
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10993479.882220260984456Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT216720.19481335510630865Clontech SMART CDS Primer II A (100% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG195220.17548663336957168TruSeq Adapter, Index 21 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT190210.17098305774626693TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)