FastQCFastQC Report
Wed 1 May 2024
EGAF00007808359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24214570
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17647857.288112074672398Clontech SMART CDS Primer II A (100% over 26bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT253810.10481705848999177Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245220.10126960751316252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)