FastQCFastQC Report
Fri 10 May 2024
EGAF00007808372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11921461
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2755472.31135261022118Clontech SMART CDS Primer II A (100% over 26bp)
NAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206410.17314153022016343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)