FastQCFastQC Report
Sun 2 Apr 2023
EGAF00007808426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24772214
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18117437.313609514272724Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8219753.318132969463287No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC906780.366047217257206Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC299270.12080874160056909Clontech SMART CDS Primer II A (96% over 26bp)
AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC295360.11923036027381324Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT288170.11632791481617268Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG276740.11171387426251042Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATC275490.11120927665165495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATAC269390.10874684031068034Clontech SMART CDS Primer II A (96% over 26bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC265890.10733396700028507Clontech SMART CDS Primer II A (96% over 26bp)
TTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC263240.1062642200652715Clontech SMART CDS Primer II A (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTG262790.10608256492536355Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT261870.10571118108377395Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTA259810.10487960422108417Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGC251520.1015331128658908Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)