FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11568602
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5493804.748888413656205Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT566210.4894368394729113TruSeq Adapter, Index 15 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA427680.36969030484409443No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA282140.2438842653589431TruSeq Adapter, Index 15 (97% over 40bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT176730.15276694625677328Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)