FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007808469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10594648
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7925927.4810602485330335Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251600.23747839475176521No Hit
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT206730.19512682252397626Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT143460.13540799090257646Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)