FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12959450
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10352537.988402285590823Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA857770.6618876572694057No Hit
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT279470.21564958389437824Clontech SMART CDS Primer II A (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT254490.19637407451705127TruSeq Adapter, Index 18 (97% over 40bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT235160.18145831806133747Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)