FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11168921
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144212712.911963474358892Clontech SMART CDS Primer II A (100% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG718010.6428642480325539TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT510790.45733155422981325TruSeq Adapter, Index 22 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAAACTCGTATG277580.2485289313085839TruSeq Adapter, Index 22 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA232360.208041582530667No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT211400.1892752218410355Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)