FastQCFastQC Report
Thu 30 Mar 2023
EGAF00007808545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9159242
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205760622.464806585523124Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA975271.0647933529870703No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT248340.27113597391574545Clontech SMART CDS Primer II A (100% over 25bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT214770.23448446934800937Clontech SMART CDS Primer II A (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG124330.13574267390249106TruSeq Adapter, Index 27 (97% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)