FastQCFastQC Report
Thu 30 Mar 2023
EGAF00007808553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8131309
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8117939.983546314621668Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA603320.7419715571010769No Hit
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT281180.3457991819029384Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT279540.34378228646826725Clontech SMART CDS Primer II A (100% over 25bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT127470.1567644274741004Clontech SMART CDS Primer II A (100% over 23bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90570.11138428019400075Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)