FastQCFastQC Report
Sun 2 Apr 2023
EGAF00007808554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23536402
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT386990916.442228510542943Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1484610.6307718571428207No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT897220.3812052496384112Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT810170.3442199874050418Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT406470.1726984438827991Clontech SMART CDS Primer II A (100% over 23bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT382680.16259069674285814Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTT249750.10611222564944292Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245360.10424702977116043Clontech SMART CDS Primer II A (100% over 22bp)
TGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244950.10407283152284703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)