FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11968282
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT183556915.336946438929164Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2046111.709610452026448No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT482840.4034330073439112Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT369420.3086658552998668Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT213960.17877252558053028Clontech SMART CDS Primer II A (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC180470.15079023037725883TruSeq Adapter, Index 11 (100% over 50bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125270.10466832248772213Clontech SMART CDS Primer II A (100% over 22bp)
AAGCAGTGGTATCAACGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTT123390.10309750388568718Clontech SMART CDS Primer II A (96% over 26bp)
TGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122150.10206143204179179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)