FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14011222
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152193810.862278821932875Clontech SMART CDS Primer II A (100% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG2560581.8275208258066284TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAACTCGTATG898840.6415143518531075TruSeq Adapter, Index 16 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA726300.518370203541133No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTATG423340.30214352466901173TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGACCTCGTATG261690.18677171769885595TruSeq Adapter, Index 16 (97% over 39bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261450.18660042642961477Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT215520.1538195597785832Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT164510.11741302792861322Clontech SMART CDS Primer II A (100% over 23bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163650.11679923421383231Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)