FastQCFastQC Report
Sat 1 Apr 2023
EGAF00007808561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18531135
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT417673522.539013395563735Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2679381.4458801363219251No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT669770.3614295616539408Clontech SMART CDS Primer II A (100% over 25bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT260220.14042313112499585Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT190630.10287011561892997Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)