FastQCFastQC Report
Thu 30 Mar 2023
EGAF00007808562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6033012
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99183016.44004686216437Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA609061.0095454807648319No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT240720.3990046762711561Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT201140.333398972188353Clontech SMART CDS Primer II A (96% over 26bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131290.21761932513974777Clontech SMART CDS Primer II A (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG121790.20187263012239992TruSeq Adapter, Index 19 (97% over 39bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT110100.18249590751684233Clontech SMART CDS Primer II A (100% over 23bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTATG73690.12214462692930164TruSeq Adapter, Index 19 (97% over 39bp)
TGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64020.10611614894848542No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61360.10170707434362804Clontech SMART CDS Primer II A (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)