FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12123560
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128434210.593769486850398Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA323750.26704202395995896No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT319490.26352820458677156Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT186060.1534697729049883Clontech SMART CDS Primer II A (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG152270.12559842158573886TruSeq Adapter, Index 20 (97% over 43bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135260.11156788929984263Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT134130.11063581984169667Clontech SMART CDS Primer II A (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)