FastQCFastQC Report
Thu 30 Mar 2023
EGAF00007808567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9164842
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT279146730.45843016169837Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6468487.057928549122833No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1086291.1852795716500077TruSeq Adapter, Index 3 (100% over 50bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT577210.6298090027083937Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT430850.4701117597008219Clontech SMART CDS Primer II A (100% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAACTCGTATGCC351290.38330175250157067TruSeq Adapter, Index 3 (98% over 50bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293160.31987458158034804Clontech SMART CDS Primer II A (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGCC115800.12635242375154968TruSeq Adapter, Index 3 (98% over 50bp)
AAGCAGTGGTATCAACGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107420.11720878548697293Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103510.11294248171436017Clontech SMART CDS Primer II A (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)