FastQCFastQC Report
Thu 30 Mar 2023
EGAF00007808574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8286304
Sequences flagged as poor quality0
Sequence length101
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT421026750.809950974523744Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7184268.67004155290465No Hit
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1184801.429829270082295Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT647440.781337493772857Clontech SMART CDS Primer II A (100% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC460080.5552294484971828TruSeq Adapter, Index 10 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342760.41364642185466527No Hit
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT307170.37069603046183197Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT281880.3401757888679923Clontech SMART CDS Primer II A (100% over 24bp)
AAGCAGTGGTATCAACGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTT248810.3002665603386021Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTATTTTTTTTTTTTTTTTTTT139000.167746681753409Clontech SMART CDS Primer II A (100% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT118280.1427415648762102Clontech SMART CDS Primer II A (100% over 23bp)
AAGCAGTGGTATCAACTCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107010.12914080873692302Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106870.1289718552445095Clontech SMART CDS Primer II A (96% over 26bp)
AAGCATTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98710.11912428025812231Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTTTTTTTTTTTTTTTTTTT95170.11485217052138082Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)