FastQCFastQC Report
Wed 8 May 2024
EGAF00007808576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9685158
Total Bases978.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT335047034.59386000723995Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1851621.9118118671889501No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT737390.7613608368598633Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT734670.7585524159750414Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT277700.2867273822481781Clontech SMART CDS Primer II A (100% over 24bp)
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT240910.24874142476560526Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTT171960.17755002035072634Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT130350.13458737585902056Clontech SMART CDS Primer II A (100% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126550.13066384668169584No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTATTTTTTTTTTTTTTTTTTT110960.11456705197788203Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102140.1054603342557757Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)