FastQCFastQC Report
Sun 5 May 2024
EGAF00007808687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16218696
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT213574113.168389123268604Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT240390.14821783452874387TruSeq Adapter, Index 14 (97% over 44bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231820.14293380922855944No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT191940.1183449026974795Clontech SMART CDS Primer II A (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)