FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007808731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007808731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12385864
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9061567.316049974390159Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1728711.39571207951258TruSeq Adapter, Index 14 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG262160.21166064797740392TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)