FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007809133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007809133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474354519
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG460725449.712681581937243TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCCCGTATG12732740.2684224454494972TruSeq Adapter, Index 7 (97% over 35bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG7496690.1580398140994626TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)