FastQCFastQC Report
Thu 6 Jul 2023
EGAF00007810356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007810356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562509242
Sequences flagged as poor quality0
Sequence length100-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108456221.928078899013005Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19311340.3433070704996523Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11939060.21224646829891552No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8860520.1575177675036315Clontech SMART CDS Primer II A (100% over 25bp)
GGAGTTTAGGGGCTCTCCCTGGTTTTAGTTGATACCAATTTAAGTAGATG7124060.12664787470283023No Hit
GTTTAGGGGCTCTCCCTGGTTTTAGTTGATACCAATTTAAGTAGATGTCA6863460.12201506193208467No Hit
GTGAAATCTGTCCCAAATCCACCGCCACTGAACCTTGATGGGACTCCACT6733450.11970381101756192No Hit
GTTGATACCAATTTAAGTAGATGTCAACAGTCTGACTTGTCCGGCAAGAG6104700.10852621689013955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)