FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007813305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007813305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462182714
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG71344281.5436379994081735TruSeq Adapter, Index 27 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCCCGTATG5748330.12437353942233331TruSeq Adapter, Index 27 (97% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)